重组Anti-Pyrophosphatase 1抗体[EPR11282] (ab181126)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11282] to Pyrophosphatase 1
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Pyrophosphatase 1抗体[EPR11282]
参阅全部 Pyrophosphatase 1 一抗 -
描述
兔单克隆抗体[EPR11282] to Pyrophosphatase 1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, HepG2, 293T or Molt4 lysate, HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11282 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181126于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/290.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Detects a band of approximately 33 kDa.
|
|
IP |
1/60.
|
说明 |
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Flow Cyt (Intra)
1/290. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 33 kDa. |
IP
1/60. |
靶标
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组织特异性
Expressed ubiquitously. -
序列相似性
Belongs to the PPase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5464 Human
- Omim: 179030 Human
- SwissProt: Q15181 Human
- Unigene: 437403 Human
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别名
- cytosolic inorganic pyrophosphatase antibody
- diphosphate phosphohydrolase antibody
- inorganic diphosphatase antibody
see all
图片
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All lanes : Anti-Pyrophosphatase 1 antibody [EPR11282] (ab181126) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution -
Intracellular Flow Cytometry analysis of HepG2 cells using ab181126at a 1/290 dilution (red) or aRabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary usedat a 1/150 dilution.
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Detection of Human Pyrophosphatase 1 by Western Blot of Immunoprecipitate. MOLT4 whole cell lysate loaded, ab181126 used for blotting immunoprecipitated Pyrophosphatase 1 at a 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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