Anti-PYK2 (phospho Y402)抗体(ab131543)
Key features and details
- Rabbit polyclonal to PYK2 (phospho Y402)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PYK2 (phospho Y402)抗体
参阅全部 PYK2 一抗 -
描述
兔多克隆抗体to PYK2 (phospho Y402) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human PYK2 (phospho Y202) conjugated to keyhole limpet haemocyanin. NP_004094.3
Database link: Q14289 -
阳性对照
- HeLa cells; Jurkat whole cell lysate (ab7899) (PMA-treated); Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab131543 was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131543于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 116 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 116 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption. -
组织特异性
Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. -
细胞定位
Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase. - Information by UniProt
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数据库链接
- Entrez Gene: 2185 Human
- Omim: 601212 Human
- SwissProt: Q14289 Human
- Unigene: 491322 Human
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别名
- CADTK antibody
- CAK-beta antibody
- CAKB antibody
see all
图片
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All lanes : Anti-PYK2 (phospho Y402) antibody (ab131543) at 1/500 dilution
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with PMA
Predicted band size: 116 kDa -
Immunoflourescent analysis of methanol-fixed HeLa cells labelling PYK2 (phospho-Y402) with ab131543 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab131543)Immunohistochemical analysis of Human breast carcinoma tissue labelling PYK2 (phospho Y402) with ab131543 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab131543 被引用在 3 文献中.
- Salazar SV et al. Alzheimer's Disease Risk Factor Pyk2 Mediates Amyloid-ß-Induced Synaptic Dysfunction and Loss. J Neurosci 39:758-772 (2019). PubMed: 30518596
- Lee S et al. Pyk2 Signaling through Graf1 and RhoA GTPase Is Required for Amyloid-ß Oligomer-Triggered Synapse Loss. J Neurosci 39:1910-1929 (2019). PubMed: 30626696
- Grenier K et al. Short mitochondrial ARF triggers Parkin/PINK1-dependent mitophagy. J Biol Chem 289:29519-30 (2014). PubMed: 25217637