AB103535

Anti-PYCR2抗体

Name: PYCR2抗体 (ab103535)| Abcam中文官网
Brand: Abcam Limited
SKU: AB103535
Price: 4629 CNY
Availability: InStock
Rating: 4 (3 reviews)

Anti-PYCR2 antibody

(3 Reviews)

(4 Publications)

Rabbit Polyclonal PYCR2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PYCR2.

查看别名

Pyrroline-5-carboxylate reductase 2, P5C reductase 2, P5CR 2, PYCR2

4 Images

Lab

Western blot - Anti-PYCR2 antibody (AB103535)

Western blot : Anti-PYCR2 antibody ab103535 staining at 1/500 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 34 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in PYCR2 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

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Western blot - Anti-PYCR2 antibody (ab103535) at 1/500 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

PYCR2 knockout MCF7 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Collaborator

Western blot - Anti-PYCR2 antibody (AB103535)

Blocking : 10% Milk

All lanes:

Western blot - Anti-PYCR2 antibody (ab103535) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 18 µg

Lane 2:

HeLa whole cell lysate at 36 µg

Lane 3:

RPE-1 whole cell lysate at 18 µg

Lane 4:

RPE-1 whole cell lysate at 36 µg

Secondary

All lanes:

LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 35 kDa

false

Exposure time: 7min

This image is courtesy of an anonymous abreview.

Unknown

Western blot - Anti-PYCR2 antibody (AB103535)

All lanes:

Western blot - Anti-PYCR2 antibody (ab103535) at 1/500 dilution

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 34 kDa,38 kDa

false

Unknown

Western blot - Anti-PYCR2 antibody (AB103535)

All lanes:

Western blot - Anti-PYCR2 antibody (ab103535) at 1/500 dilution

Lane 1:

PYCR2 transfected 293T cell line lysate at 25 µg

Lane 2:

Non-transfected 293T cell line lysate at 25 µg

Predicted band size: 20 kDa,34 kDa,39 kDa,81 kDa,92 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

Recombinant Full Length Protein corresponding to Human PYCR2.

Q96C36

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PYCR2 also known as Pyrroline-5-carboxylate reductase 2 is an enzyme involved in proline biosynthesis. It has a molecular mass of approximately 31 kDa. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline an amino acid important for protein synthesis and cellular stress responses. Expressed broadly in human tissues PYCR2 shows notable expression in the liver brain and various muscle tissues indicating its wide functional relevance across different physiological systems.

Biological function summary

The enzyme participates in maintaining proline levels and contributes to cellular osmotic balance and protection against oxidative stress. It is not typically part of a larger protein complex but interacts with other metabolic enzymes that support similar stress response pathways. By catalyzing an important step in the proline biosynthesis pathway PYCR2 helps sustain cellular redox states and protein turnover rates under stressful conditions.

Pathways

PYCR2 plays a significant role in the proline metabolism pathway and glutamate pathway. The functioning of this enzyme in these pathways supports the synthesis and recycling of proline which is important for cell survival under osmotic and oxidative stress conditions. The enzyme cooperates indirectly with proteins such as glutamate dehydrogenase facilitating the metabolic flux through interconnected pathways that manage cellular nitrogen balance.

Mutations in PYCR2 have been associated with conditions such as Hypomyelinating Leukodystrophy 10 and Proline Metabolism Disorder. These conditions often showcase symptoms related to central nervous system abnormalities and metabolic imbalances. The disruptions in PYCR2 activity may also alter interactions with proteins involved in these pathways such as Δ1-Pyrroline-5-carboxylate synthase thereby exacerbating the disease states.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H (PubMed : 23024808, PubMed : 2722838, PubMed : 6894153). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed : 23024808, PubMed : 2722838, PubMed : 6894153). Involved in cellular response to oxidative stress (PubMed : 25865492). In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+) (PubMed : 2722838, PubMed : 6894153).

See full target information PYCR2

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 41:45 PubMed35105345

2022

Pyrroline-5-Carboxylate Reductase 1: a novel target for sensitizing multiple myeloma cells to bortezomib by inhibition of PRAS40-mediated protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Inge Oudaert,Hatice Satilmis,Philip Vlummens,Wouter De Brouwer,Anke Maes,Dirk Hose,Elke De Bruyne,Bart Ghesquière,Karin Vanderkerken,Kim De Veirman,Eline Menu

Disease markers 2021:9950663 PubMed34512817

2021

Pyrroline-5-Carboxylate Reductase-2 Promotes Colorectal Cancer Progression via Activating PI3K/AKT/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yin,Xiaoxia Huang,Yi Xuan

Oncology letters 19:3815-3826 PubMed32391095

2020

Identification of potential cervical cancer serum biomarkers in Thai patients.

Applications

Unspecified application

Species

Unspecified reactive species

Siriporn Keeratichamroen,Pantipa Subhasitanont,Daranee Chokchaichamnankit,Churat Weeraphan,Kittirat Saharat,Narongrit Sritana,Nuttavut Kantathavorn,Kriangpol Wiriyaukaradecha,Thaniya Sricharunrat,N Monique Paricharttanakul,Chirayu Auewarakul,Jisnuson Svasti,Chantragan Srisomsap

Regenerative therapy 6:83-89 PubMed30271842

2017

Cytological character of mini pig mesenchymal stromal cells from various tissues and the attempt of cell sheet formation.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuyuki Kaibuchi,Takanori Iwata,Satoru Onizuka,Kosei Yano,Masayuki Yamato,Teruo Okano,Tomohiro Ando

View all publications

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Anti-PYCR2抗体

Anti-PYCR2 antibody

Western blot - Anti-PYCR2 antibody (AB103535)

Western blot - Anti-PYCR2 antibody (AB103535)

Western blot - Anti-PYCR2 antibody (AB103535)

Western blot - Anti-PYCR2 antibody (AB103535)