Anti-PYCR1抗体- C-terminal (ab226340)
Key features and details
- Rabbit polyclonal to PYCR1 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PYCR1抗体- C-terminal
参阅全部 PYCR1 一抗 -
描述
兔多克隆抗体to PYCR1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human PYCR1 aa 269-319 (C terminal). The exact sequence is proprietary.
Database link: P32322 -
阳性对照
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226340 was affinity purified using an epitope specific to PYCR1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226340于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 33 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 33 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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相关性
PYCR1 is an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. -
细胞定位
Mitochondrial -
数据库链接
- Entrez Gene: 454977 Chimpanzee
- Entrez Gene: 539606 Cow
- Entrez Gene: 101146033 Gorilla
- Entrez Gene: 5831 Human
- Entrez Gene: 715182 Rhesus monkey
- Omim: 179035 Human
- SwissProt: Q58DT4 Cow
- SwissProt: P32322 Human
see all -
别名
- P5C antibody
- P5C reductase 1 antibody
- P5C reductase antibody
see all
图片
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Western blot - Anti-PYCR1 antibody - C-terminal (ab226340)All lanes : Anti-PYCR1 antibody - C-terminal (ab226340) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 30 secondsCell lysates were prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-PYCR1 antibody - C-terminal (ab226340)PYCR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226340 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226340 at 1 µg/ml.
Lane 1: ab226340 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab226340 被引用在 2 文献中.
- Song W et al. Antisense lncRNA-RP11-498C9.13 promotes bladder cancer progression by enhancing reactive oxygen species-induced mitophagy. J Gene Med 25:e3527 (2023). PubMed: 37382425
- Song W et al. Dysregulation of USP18/FTO/PYCR1 signaling network promotes bladder cancer development and progression. Aging (Albany NY) 13:3909-3925 (2021). PubMed: 33461172