AB307596

重组Anti-PVRIG/CD112R抗体[EPR26274-202] - BSA and Azide free

Anti-PVRIG/CD112R antibody [EPR26274-202] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PVRIG/CD112R antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

查看别名

C7orf15, PVRIG, Transmembrane protein PVRIG, CD112 receptor, Poliovirus receptor-related immunoglobulin domain-containing protein, CD112R

2 Images

Supplier Data

Western blot - Anti-PVRIG/CD112R antibody [EPR26274-202] - BSA and Azide free (AB307596)

This data was developed using ab307595, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure times : Lane 1 : 10 seconds Lane 2 and 3 : 1 second

All lanes:

Western blot - Anti-PVRIG/CD112R antibody [EPR26274-202] (<a href='/products/primary-antibodies/pvrig-cd112r-antibody-epr26274-202-ab307595'>ab307595</a>) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Mouse lymph node tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/2000 dilution

Observed band size: 30 kDa

false

Supplier Data

Western blot - Anti-PVRIG/CD112R antibody [EPR26274-202] - BSA and Azide free (AB307596)

This data was developed using ab307595, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : HeLa Exposure time : 180 seconds.

All lanes:

Western blot - Anti-PVRIG/CD112R antibody [EPR26274-202] (<a href='/products/primary-antibodies/pvrig-cd112r-antibody-epr26274-202-ab307595'>ab307595</a>) at 1/1000 dilution

Lane 1:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

Unconjugated

Anti-PVRIG/CD112R antibody [EPR26274-202]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR26274-202

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The PVRIG also known as CD112R is a cell surface receptor involved in immune responses. It has a molecular mass of approximately 38 kDa. Expressed mainly in immune cells including T cells and natural killer (NK) cells PVRIG binds with high affinity to its ligand CD112. This interaction modulates immune cell function by transmitting inhibitory signals that downregulate immune activation serving as a potential immune checkpoint.

Biological function summary

PVRIG impacts immune cell behavior and is part of the inhibitory receptor family. Its primary role is to fine-tune the immune response by decreasing overactivation helping to maintain immune homeostasis. PVRIG interacts with other immune inhibitory receptors like TIGIT and PD-1 forming a complex network that suppresses overactive T cell responses. This process is important for preventing autoimmune reactions while allowing normal immune surveillance.

Pathways

PVRIG participates in immune checkpoint pathways that influence T cell regulation. It plays a role in the immune checkpoint receptor pathway which is pivotal for controlling immune tolerance and immune-mediated inflammation. Within these pathways PVRIG works closely with other receptors like PD-1 forming a blockade that maintains the balance of immune activation and inhibition and participates in the immune synapse during antigen presentation.

PVRIG is relevant to cancer and autoimmune diseases. Its expression on T cells can be upregulated in tumor environments contributing to immune escape in cancers like melanoma. The PVRIG-CD112 interaction competes with other pathways such as TIGIT and CD112 further impacting tumor immune evasion. In autoimmune diseases PVRIG may prevent excessive immune responses linking it to conditions like rheumatoid arthritis where altered immune regulation occurs.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

See full target information PVRIG

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重组Anti-PVRIG/CD112R抗体[EPR26274-202] - BSA and Azide free