Anti-PURA抗体(ab79936)
Key features and details
- Rabbit polyclonal to PURA
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-PURA抗体
参阅全部 PURA 一抗 -
描述
兔多克隆抗体to PURA -
宿主
Rabbit -
特异性
From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab129206.
-
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Sheep
-
免疫原
Synthetic peptide corresponding to Human PURA aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab88160) -
阳性对照
- This antibody gave a positive signal in Human Brain Tissue Lysate
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79936于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).
|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa). |
|
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
-
功能
This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination. -
序列相似性
Belongs to the PUR DNA-binding protein family. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 5813 Human
- Entrez Gene: 19290 Mouse
- Omim: 600473 Human
- SwissProt: Q00577 Human
- SwissProt: P42669 Mouse
- Unigene: 443121 Human
- Unigene: 231802 Mouse
- Unigene: 482241 Mouse
-
别名
- PUR ALPHA antibody
- PUR1 antibody
- purA antibody
see all
图片
-
Western blot - Anti-PURA antibody (ab79936)Anti-PURA antibody (ab79936) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?PURA contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Furthermore, other commercially available antibodies react with a target at approximately 42 kDa.
-
Immunocytochemistry/ Immunofluorescence - Anti-PURA antibody (ab79936)ICC/IF image of ab79936 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79936, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293. HepG2, MCF-7 cells at 5µg/ml.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (10)
ab79936 被引用在 10 文献中.
- Anadolu MN et al. Ribosomes in RNA Granules Are Stalled on mRNA Sequences That Are Consensus Sites for FMRP Association. J Neurosci 43:2440-2459 (2023). PubMed: 36849416
- Hardt R et al. Proteomic investigation of neural stem cell to oligodendrocyte precursor cell differentiation reveals phosphorylation-dependent Dclk1 processing. Cell Mol Life Sci 80:260 (2023). PubMed: 37594553
- Sun Y et al. PURa Promotes the Transcriptional Activation of PCK2 in Oesophageal Squamous Cell Carcinoma Cells. Genes (Basel) 11:N/A (2020). PubMed: 33142842
- Swinnen B et al. A zebrafish model for C9orf72 ALS reveals RNA toxicity as a pathogenic mechanism. Acta Neuropathol 135:427-443 (2018). PubMed: 29302778
- Moldovan JB & Moran JV The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition. PLoS Genet 11:e1005121 (2015). PubMed: 25951186
- Di Salvio M et al. Pur-alpha functionally interacts with FUS carrying ALS-associated mutations. Cell Death Dis 6:e1943 (2015). PubMed: 26492376
- Rossi S et al. Nuclear accumulation of mRNAs underlies G4C2-repeat-induced translational repression in a cellular model of C9orf72 ALS. J Cell Sci 128:1787-99 (2015). PubMed: 25788698
- Hay CW et al. An Sp1 Modulated Regulatory Region Unique to Higher Primates Regulates Human Androgen Receptor Promoter Activity in Prostate Cancer Cells. PLoS One 10:e0139990 (2015). WB . PubMed: 26448047
- Schreiber J et al. Ubiquitin ligase TRIM3 controls hippocampal plasticity and learning by regulating synaptic ?-actin levels. J Cell Biol 211:569-86 (2015). PubMed: 26527743
- Xu Z et al. Expanded GGGGCC repeat RNA associated with amyotrophic lateral sclerosis and frontotemporal dementia causes neurodegeneration. Proc Natl Acad Sci U S A 110:7778-83 (2013). WB . PubMed: 23553836