Anti-PURA抗体(ab125200)
Key features and details
- Rabbit polyclonal to PURA
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PURA抗体
参阅全部 PURA 一抗 -
描述
兔多克隆抗体to PURA -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within amino acids 100-150 of Human PURA (NP_005850.1).
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阳性对照
- HeLa whole cell lysate (ab150035).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab125200 was affinity purified using an epitope specific to PURA immobilized on a solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125200于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination. -
序列相似性
Belongs to the PUR DNA-binding protein family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5813 Human
- Entrez Gene: 19290 Mouse
- Entrez Gene: 307498 Rat
- Omim: 600473 Human
- SwissProt: Q00577 Human
- SwissProt: P42669 Mouse
- SwissProt: P86252 Rat
- Unigene: 443121 Human
see all -
别名
- PUR ALPHA antibody
- PUR1 antibody
- purA antibody
see all
图片
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Immunoprecipitation - Anti-PURA antibody (ab125200)Detection of PURA by Western Blot of Immunprecipitate. Lane 1; an antibody to PURA at 1µg/ml staining PURA in HeLa whole cell lysate immunoprecipitated using ab125200 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab125200 被引用在 3 文献中.
- Tian L et al. Forming cytoplasmic stress granules PURα suppresses mRNA translation initiation of IGFBP3 to promote esophageal squamous cell carcinoma progression. Oncogene 41:4336-4348 (2022). PubMed: 35945453
- Sun Y et al. PURa Promotes the Transcriptional Activation of PCK2 in Oesophageal Squamous Cell Carcinoma Cells. Genes (Basel) 11:N/A (2020). PubMed: 33142842
- Chang KC et al. MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression. Nat Commun 11:6438 (2020). PubMed: 33353933