AB130999

Anti-Pur B抗体

Name: Pur B抗体 (ab130999)| Abcam中文官网
Brand: Abcam Limited
SKU: AB130999
Price: 4744 CNY
Availability: InStock

Anti-Pur B antibody

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(2 Publications)

Rabbit Polyclonal Pur B antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PURB aa 250 to C-terminus.

查看别名

Transcriptional regulator protein Pur-beta, Purine-rich element-binding protein B, PURB

1 Images

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Immunoprecipitation - Anti-Pur B antibody (AB130999)

Detection of Pur B by Western Blot of Immunprecipitate.
ab130999 at 1µg/ml staining Pur B in Jurkat whole cell lysate immunoprecipitated using ab1130999 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-Pur B antibody (ab130999)

Predicted band size: 33 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human PURB aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96QR8

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab130999 is affinity purified using an epitope specific to Pur B immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle|Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pur B also known as purine-rich element-binding protein B is involved in DNA binding and transcriptional regulation. It is a 40 kDa protein localized in the nucleus and highly expressed in tissues with active cellular proliferation like the brain and the heart. Pur B associates with the purine-rich single-stranded DNA influencing the function and stability of genetic elements within these tissues.

Biological function summary

Pur B plays a significant role in cell growth and differentiation. It forms part of a multi-protein complex essential for regulating the expression of genes vital for cellular processes. The complex influences transcriptional activity altering how certain genes are turned on or off which ultimately impacts cellular function and development.

Pathways

Pur B is integral to the regulation of transcription and nucleic acid processes. It is involved in pathways like the response to stress and regulation of apoptosis. Within these pathways Pur B interacts with other transcription factors such as Pur A contributing to the precise control of gene expression essential for maintaining cellular integrity and responding to physiological changes.

Pur B displays an association with neurodegenerative diseases and cancer. Dysregulation of Pur B levels or function links to Alzheimer’s disease where it affects neural development and function often alongside Pur A. Additionally abnormal Pur B activity is observed in certain cancers potentially due to its role in gene transcription involved in cell proliferation where it might interact with oncogenic factors exacerbating tumor progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional regulator which can act as an activator or a repressor. Represses the transcription of ACTA2 in fibroblasts and smooth muscle cells via its ability to interact with the purine-rich strand of a MCAT- containing element in the 5' flanking region of the gene. Represses the transcription of MYOCD, capable of repressing all isoforms of MYOCD but the magnitude of the repressive effects is most notable for the SMC- specific isoforms. Promotes hepatic glucose production by activating the transcription of ADCY6, leading to cAMP accumulation, increased PKA activity, CREB activation, and increased transcription of PCK1 and G6PC genes (By similarity). Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene (PubMed : 1448097). Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity).

See full target information PURB

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40548942

2025

The lincRNA Pantr1 is a FOXG1 target gene conferring site-specific chromatin binding of FOXG1.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Gather,Tudor Rauleac,Ipek Akol,Ganeshkumar Arumugam,Camila L Fullio,Teresa Müller,Dimitrios Kleidonas,Ruth Geiss-Friedlander,Andre Fischer,Andreas Vlachos,Rolf Backofen,Tanja Vogel

International journal of biological sciences 20:1669-1687 PubMed38481813

2024

Tubule-specific cyclin-dependent kinase 12 knockdown potentiates kidney injury through transcriptional elongation defects.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Lin Zhang,Tao-Tao Tang,Wei-Jie Ni,Zhong-Tang Li,Liang-Yun-Zi Jiang,Yao Wang,Xuan Zhou,Jing-Yuan Cao,Qing Yin,Wei Jiang,Ya-Jie Zhao,Wei-Hua Gan,Ai-Qing Zhang,Zuo-Lin Li,Yi Wen,Lin-Li Lv,Bi-Cheng Liu,Bin Wang

View all publications

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Anti-Pur B抗体

Anti-Pur B antibody

Immunoprecipitation - Anti-Pur B antibody (AB130999)