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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation

Anti-Pumilio 1抗体(ab3717)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (3)

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Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
  • Western blot - Anti-Pumilio 1 antibody (ab3717)
  • Western blot - Anti-Pumilio 1 antibody (ab3717)

Key features and details

  • Goat polyclonal to Pumilio 1
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Pumilio 1抗体
    参阅全部 Pumilio 1 一抗
  • 描述

    山羊多克隆抗体to Pumilio 1
  • 宿主

    Goat
  • 经测试应用

    适用于: IP, WBmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.

  • 阳性对照

    • WB: NIH/3T3, HEK293T, A549, K562 whole cell lysate; IP: HEK293T
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3717于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at 2-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).
说明
IP
Use at 2-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).

靶标

  • Target information above from: UniProt accession Q14671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 535450 Cow
    • Entrez Gene: 9698 Human
    • Entrez Gene: 80912 Mouse
    • Entrez Gene: 362609 Rat
    • Omim: 607204 Human
    • SwissProt: Q14671 Human
    • SwissProt: Q80U78 Mouse
    • SwissProt: Q5R5X3 Orangutan
    • Unigene: 281707 Human
    • Unigene: 440206 Mouse
    see all
  • 形式

    Cytoplasm
  • 别名

    • HsPUM antibody
    • KIAA0099 antibody
    • PUM 1 antibody
    • PUM L1 antibody
    • Pum1 antibody
    • PUM1_HUMAN antibody
    • PUMH 1 antibody
    • PUMH antibody
    • PUMH1 antibody
    • pumilio homolog 1 (Drosophila) antibody
    • Pumilio homolog 1 antibody
    • Pumilio-1 antibody
    • Pumilio1 antibody
    • PUML 1 antibody
    • PUML1 antibody
    see all

图片

  • Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
    Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)

    Pumilio 1 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3717 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3717 at 0.1 µg/mL.

    Lane 1: rabbit anti-Pumillio 1 antibody IP in HEK293T whole cell lysate.

    Lane 2: ab3717 IP in HEK293T whole cell lysate.

    Lane 3: ab3717 IP in HEK293T whole cell lysate

    Lane 4: Control IgG in HEK293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 seconds

  • Western blot - Anti-Pumilio 1 antibody (ab3717)
    Western blot - Anti-Pumilio 1 antibody (ab3717)
    All lanes : Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : A549 whole cell lysate
    Lane 3 : K562 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated rabbit anti-goat IgG

    Predicted band size: 126 kDa


    Exposure time: 10 seconds
  • Western blot - Anti-Pumilio 1 antibody (ab3717)
    Western blot - Anti-Pumilio 1 antibody (ab3717)
    Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/ml + NIH/3T3 whole cell lysate at 50 µg

    Secondary
    HRP-conjugated rabbit anti-goat IgG

    Predicted band size: 126 kDa


    Exposure time: 10 seconds

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (3)

发表研究结果有使用 ab3717?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3717 被引用在 3 文献中.

  • Sajek M  et al. PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cell Mol Life Sci 76:147-161 (2019). PubMed: 30269240
  • Janecki DM  et al. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget 9:32466-32477 (2018). PubMed: 30197756
  • Marrero E  et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). IHC-Fr ; Mouse . PubMed: 21865157

客户评价及客户问答

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Question

Customer's question: Do you know whether ab3717 cross reacts with Pum2. If you do not know, could you send me the peptide sequence to see if it is likely to cross react. Thank you

Read More

Abcam community

Verified customer

Asked on Nov 19 2003

Answer

ab3717 does not cross react with Pum2.

Read More

Abcam Scientific Support

回复于 Nov 24 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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