Anti-PUMA抗体(ab9643)
Key features and details
- Rabbit polyclonal to PUMA
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PUMA抗体
参阅全部 PUMA 一抗 -
描述
兔多克隆抗体to PUMA -
宿主
Rabbit -
特异性
At least 2 isoforms are known to exist; this antibody will detect both isoforms. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human PUMA aa 150-250 (C terminal).
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常规说明
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (9) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Can be blocked with PUMA peptide (180/193) (ab9644). A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Can be blocked with PUMA peptide (180/193) (ab9644). A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form. |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Essential mediator of p53-dependent and p53-independent apoptosis. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the Bcl-2 family. -
细胞定位
Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release. - Information by UniProt
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数据库链接
- Entrez Gene: 27113 Human
- Entrez Gene: 170770 Mouse
- Omim: 605854 Human
- SwissProt: Q9BXH1 Human
- SwissProt: Q99ML1 Mouse
- Unigene: 467020 Human
- Unigene: 7660 Mouse
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别名
- BBC 3 antibody
- Bbc3 antibody
- BBC3_HUMAN antibody
see all
图片
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Western blot - Anti-PUMA antibody (ab9643)All lanes : Anti-PUMA antibody (ab9643) at 2 µg/ml
Lane 1 : HEK293 cells were transfected with control siRNAs with control siRNAs
Lane 2 : HEK293 cells were transfected with PUMA siRNAs with PUMA siRNA
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionBeta-actin (1 μg/mL) and GAPDH (0.02 μg/mL). Incubation time: 1 hour at Room Temperature in 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9643)Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9643 at 2 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells.
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9643)ICC/IF image of ab9643 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9643, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9643)Immunocytochemical analysis of K562 cells labeling PUMA with ab9643 at 1 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Western blot - Anti-PUMA antibody (ab9643)All lanes : Anti-PUMA antibody (ab9643) at 2 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : NIH3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Western blot - Anti-PUMA antibody (ab9643)This image is courtesy of an anonymous AbreviewAnti-PUMA antibody (ab9643) at 1/1000 dilution + HeLa whole cell lysate
Secondary
Alexa Fluor 680-conjugated goat anti-rabbit IgG polyclonal at 1/1 dilution
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible non-specific binding), 42 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Exposure time: 5 secondsBlocked with 5% milk for 1 hour.
Incubated with the primary antibody for 18 hours.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (130)
ab9643 被引用在 130 文献中.
- Wei Z et al. Inhibition of secretory leukocyte protease inhibitor (SLPI) promotes the PUMA-mediated apoptosis and chemosensitivity to cisplatin in colorectal cancer cells. Discov Oncol 14:1 (2023). PubMed: 36595102
- Mu M et al. 3-Bromopyruvate overcomes cetuximab resistance in human colorectal cancer cells by inducing autophagy-dependent ferroptosis. Cancer Gene Ther 30:1414-1425 (2023). PubMed: 37558749
- Yao F et al. Deletion of ARGLU1 causes global defects in alternative splicing in vivo and mouse cortical malformations primarily via apoptosis. Cell Death Dis 14:543 (2023). PubMed: 37612280
- Lei M et al. Molecular mechanism and therapeutic potential of HDAC9 in intervertebral disc degeneration. Cell Mol Biol Lett 28:104 (2023). PubMed: 38093179
- Huang Y et al. Targeting Glycolysis in Alloreactive T Cells to Prevent Acute Graft-Versus-Host Disease While Preserving Graft-Versus-Leukemia Effect. Front Immunol 13:751296 (2022). PubMed: 35296079