Anti-PUMA抗体(ab54288)
Key features and details
- Rabbit polyclonal to PUMA
- Suitable for: WB, ICC/IF, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PUMA抗体
参阅全部 PUMA 一抗 -
描述
兔多克隆抗体to PUMA -
宿主
Rabbit -
特异性
Detects a 23 kD protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16kd band may be seen in some instances, possibly corresponding to PUMAß. -
经测试应用
适用于: WB, ICC/IF, ICCmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human PUMA (C terminal).
Database link: Q9BXH1 -
阳性对照
- ICC/IF: Heat Shocked HeLa cells. ICC: Human K562 cells. WB: Human K562 cell lysate, Mouse 3T3 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 21 kDa). A 16 kDa band may be seen in some instances, possibly corresponding to PUMAß.
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ICC/IF |
1/100.
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ICC |
1/100.
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说明 |
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WB
1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 21 kDa). A 16 kDa band may be seen in some instances, possibly corresponding to PUMAß. |
ICC/IF
1/100. |
ICC
1/100. |
靶标
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功能
Essential mediator of p53-dependent and p53-independent apoptosis. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the Bcl-2 family. -
细胞定位
Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release. - Information by UniProt
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数据库链接
- Entrez Gene: 27113 Human
- Entrez Gene: 170770 Mouse
- Omim: 605854 Human
- SwissProt: Q9BXH1 Human
- SwissProt: Q99ML1 Mouse
- Unigene: 467020 Human
- Unigene: 7660 Mouse
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别名
- BBC 3 antibody
- Bbc3 antibody
- BBC3_HUMAN antibody
see all
图片
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Immunocytochemistry/Immunofluorescence analysis using ab54288 at 1/125 for 12 hours at 4°C in Heat Shocked HeLa Cells. Sfixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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All lanes : Anti-PUMA antibody (ab54288) at 1/1000 dilution
Lane 1 : Cell lysates prepared form human K562 cells
Lane 2 : Cell lysates prepared from mouse 3T3 cells
Predicted band size: 21 kDa -
Immunocytochemistry/Immunofluorescence analysis using at ab54288 1/125 for 12 hours at 4°C. Tissue: Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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ab54288 at 1/100 dilution staining PUMA in human K562 cells by Immunocytochemistry.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (13)
ab54288 被引用在 13 文献中.
- Carr D et al. Multiple roles for Bcl-3 in mammary gland branching, stromal collagen invasion, involution and tumor pathology. Breast Cancer Res 24:40 (2022). PubMed: 35681213
- Alotaibi M et al. Alleviation of cisplatin-induced neuropathic pain, neuronal apoptosis, and systemic inflammation in mice by rapamycin. Front Aging Neurosci 14:891593 (2022). PubMed: 36248001
- Lapresa R et al. Amyloid-ß promotes neurotoxicity by Cdk5-induced p53 stabilization. Neuropharmacology 146:19-27 (2019). PubMed: 30452955
- Ramos-Araque ME et al. The Neuronal Ischemic Tolerance Is Conditioned by the Tp53 Arg72Pro Polymorphism. Transl Stroke Res 10:204-215 (2019). PubMed: 29687302
- Vecino R et al. The MDM2-p53 pathway is involved in preconditioning-induced neuronal tolerance to ischemia. Sci Rep 8:1610 (2018). PubMed: 29371613