Anti-PUF60/FIR抗体(ab225707)
Key features and details
- Rabbit polyclonal to PUF60/FIR
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PUF60/FIR抗体
参阅全部 PUF60/FIR 一抗 -
描述
兔多克隆抗体to PUF60/FIR -
宿主
Rabbit -
经测试应用
适用于: IP, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Orangutan
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免疫原
Synthetic peptide within Human PUF60/FIR aa 400-450. The exact sequence is proprietary. NP_510965.1
Database link: Q9UHX1 -
阳性对照
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian cancer and mouse teratoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225707于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
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| IHC-P |
1/200 - 1/1000.
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| 说明 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 60 kDa. |
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IHC-P
1/200 - 1/1000. |
靶标
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功能
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA. -
组织特异性
Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. -
序列相似性
Belongs to the RRM half pint family.
Contains 3 RRM (RNA recognition motif) domains. -
结构域
The third RNA recognition motif, called PUMP domain, is atypical and may rather mediate homodimerization and/or protein-protein interactions. -
细胞定位
Nucleus. Colocalizes partially with TROVE2. - Information by UniProt
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数据库链接
- Entrez Gene: 512824 Cow
- Entrez Gene: 22827 Human
- Entrez Gene: 67959 Mouse
- Entrez Gene: 100174042 Orangutan
- Entrez Gene: 84401 Rat
- Omim: 604819 Human
- SwissProt: Q2HJG2 Cow
- SwissProt: Q9UHX1 Human
see all -
别名
- 60 kDa poly(U)-binding-splicing factor antibody
- FBP-interacting repressor antibody
- FIR antibody
see all
图片
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Western blot - Anti-PUF60/FIR antibody (ab225707)All lanes : Anti-PUF60/FIR antibody (ab225707) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUF60/FIR antibody (ab225707)Formalin fixed, paraffin embedded human colon carcinoma tissue stained for PUF60/FIR with ab225707 (1/200 dilution) in immunohistochemical analysis. DAB detection.
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Immunoprecipitation - Anti-PUF60/FIR antibody (ab225707)Whole cell lysate from HeLa (human epithelial cell line from cervix adenocarcinoma) cells (1 mg for IP, 20% of IP loaded). ab225707 used for for WB at 0.4 µg/mg and IP at 3 µg/mg lysate.
Lane 1: ab225707 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence 3 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUF60/FIR antibody (ab225707)Formalin fixed, paraffin embedded mouse teratoma tissue stained for PUF60/FIR with ab225707 (1/200 dilution) in immunohistochemical analysis. DAB detection.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225707 尚未被引用在任何文献中。