AB22819

Anti-PUF60/FIR抗体

Name: PUF60/FIR抗体 (ab22819)| Abcam中文官网
Brand: Abcam Limited
SKU: AB22819
Price: 4606 CNY
Availability: InStock

Anti-PUF60/FIR antibody

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(3 Publications)

Goat Polyclonal PUF60/FIR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PUF60 aa 500 to C-terminus.

查看别名

FIR, ROBPI, SIAHBP1, PUF60, Poly(U)-binding-splicing factor PUF60, 60 kDa poly(U)-binding-splicing factor, FUSE-binding protein-interacting repressor, Ro-binding protein 1, Siah-binding protein 1, FBP-interacting repressor, RoBP1, Siah-BP1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-PUF60/FIR antibody (AB22819)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PUF60/FIR antibody (AB22819)

ICC/IF image of ab22819 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22819, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human PUF60 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UHX1

特异性

ab22819 recognises both reported human isoforms of PUF60/FIR

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab22819 has bee purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PUF60 also known as Far-Upstream Element-Binding Protein 1 (FUBP1) or FIR (FUSE-Binding Protein-Interacting Repressor) is a protein with a molecular mass of about 60 kDa. It expresses in various tissues with significant levels detectable in the nucleus where it functions. This protein plays a role in RNA processing and gene expression regulation by binding to nucleic acids.

Biological function summary

PUF60/FIR is essential for mRNA processing. It is part of the spliceosome complex where it interacts with other splicing factors to facilitate pre-mRNA splicing. PUF60/FIR regulates alternative splicing events which can influence gene expression profiles enabling response to different physiological conditions. It connects with the regulation of cell growth and differentiation through its splicing activities.

Pathways

PUF60/FIR participates in the regulation of transcription and mRNA processing pathways. These pathways include the transcription regulation of MYC a critical protein for cell proliferation and apoptosis. PUF60/FIR modulates the splicing and transcription of RNA polymerase II-dependent genes functioning in interaction with other proteins such as SF3B1 and hnRNP H. This interaction influences the transcriptional activity of gene networks involved in cell cycle progression and survival pathways.

PUF60/FIR has an association with cancer and developmental disorders. Altered expression or mutations in PUF60/FIR link to certain cancers like colorectal cancer due to dysregulation of splicing events. It is also implicated in Verheij syndrome a developmental disorder attributed to haploinsufficiency of PUF60/FIR. In these contexts it interacts with proteins involved in cell cycle regulation and apoptosis like p53 highlighting its broader role in maintaining proper cellular function and development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with RO60. Binds to poly(U) RNA.

See full target information PUF60

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 31:1884-1908 PubMed35094084

2022

Deregulation of microtubule organization and RNA metabolism in Arx models for lissencephaly and developmental epileptic encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Denise Drongitis,Marianna Caterino,Lucia Verrillo,Pamela Santonicola,Michele Costanzo,Loredana Poeta,Benedetta Attianese,Adriano Barra,Gaetano Terrone,Maria Brigida Lioi,Simona Paladino,Elia Di Schiavi,Valerio Costa,Margherita Ruoppolo,Maria Giuseppina Miano

Cancer management and research 11:821-830 PubMed30697074

2019

PUF60 accelerates the progression of breast cancer through downregulation of PTEN expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dongying Sun,Wei Lei,Xiaodong Hou,Hui Li,Wenlu Ni

The Journal of biological chemistry 284:630-639 PubMed18974054

2008

Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60.

Applications

Species

Unspecified reactive species

Lorenzo Corsini,Michael Hothorn,Gunter Stier,Vladimir Rybin,Klaus Scheffzek,Toby J Gibson,Michael Sattler

View all publications

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Anti-PUF60/FIR抗体

Anti-PUF60/FIR antibody

Immunocytochemistry/ Immunofluorescence - Anti-PUF60/FIR antibody (AB22819)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性

反应性数据

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (3)

Abcam Product Promise

Anti-PUF60/FIR抗体

Anti-PUF60/FIR antibody

Immunocytochemistry/ Immunofluorescence - Anti-PUF60/FIR antibody (AB22819)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性

替代产品

Primary Antibodies

Primary Antibodies

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (3)

Abcam Product Promise