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重组RabMAb

重组Anti-PU.1/Spi1抗体[EPR3158Y] (ab76543)

Reviews (3)Q&A (1)References (18)
Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)
  • Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3158Y] to PU.1/Spi1
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

概述

  • 产品名称

    Anti-PU.1/Spi1抗体[EPR3158Y]
    参阅全部 PU.1/Spi1 一抗

  • 描述

    兔单克隆抗体[EPR3158Y] to PU.1/Spi1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details
    不适用于: Flow Cyt (Intra) or ICC/IF

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human PU.1/Spi1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P17947

  • 阳性对照

    • WB: Daudi, THP-1 and Raji Whole cell lysate IHC: human tonsil tissue

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76543于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use: 1/100.00000 - 1/250.00000 
WB (2)
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 31 kDa).

For unpurified use: 1/5000.00000 - 1/20000.00000
说明
IHC-P
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use: 1/100.00000 - 1/250.00000 
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 31 kDa).

For unpurified use: 1/5000.00000 - 1/20000.00000
应用说明
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

靶标

  • 功能

    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.

  • 序列相似性

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • transcription factor spi1 antibody
      • 31 kDa Transforming Protein antibody
      • 31 kDa-transforming protein antibody
      • cb1086 antibody
      • Hematopoietic transcription factor PU.1 antibody
      • OF antibody
      • oncogene spi1 antibody
      • PU.1 antibody
      • SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
      • SFPI1 antibody
      • si:by184l24.2 antibody
      • SPI 1 antibody
      • SPI 1 proto oncogene antibody
      • SPI A antibody
      • Spi1 antibody
      • SPI1_HUMAN antibody
      • Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody
      • Spleen focus forming virus proviral integration oncogene spi1 antibody
      • Transcription factor PU.1 antibody
      see all

    图片

    • Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)
      Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)
      All lanes : Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/1000 dilution (Purified)

      Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
      Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
      Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 37-42 kDa why is the actual band size different from the predicted?



      PU.1 is a 37-42 kDa member of the ets family of transcription factors. The molecule weight observed is consistent with what has been described in the literature (PMID: 28362429, 31586032, 28586009).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling PU.1/Spi1 with Purified ab76543 at 1:3000 dilution (0.035 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
    • Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)
      Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)

    文献 (18)

    发表研究结果有使用 ab76543？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab76543 被引用在 18 文献中.

    • Chen SW & Wong YH Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors. Methods Mol Biol 2683:53-68 (2023). PubMed: 37300766
    • Huang J  et al. Comprehensive Analysis of Immune Implications and Prognostic Value of SPI1 in Gastric Cancer. Front Oncol 12:820568 (2022). PubMed: 35237521
    • Patrick DM  et al. Isolevuglandins disrupt PU.1-mediated C1q expression and promote autoimmunity and hypertension in systemic lupus erythematosus. JCI Insight 7:N/A (2022). PubMed: 35608913
    • Porras DP  et al. Challenges in Cell Fate Acquisition to Scid-Repopulating Activity from Hemogenic Endothelium of hiPSCs Derived from AML Patients Using Forced Transcription Factor Expression. Cells 11:N/A (2022). PubMed: 35741044
    • Tian J  et al. MIR503HG impeded ovarian cancer progression by interacting with SPI1 and preventing TMEFF1 transcription. Aging (Albany NY) 14:5390-5405 (2022). PubMed: 35771155
    View all Publications for this product

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-PU.1/Spi1 antibody [EPR3158Y]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (AML Cells (MV411))
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    AML Cells (MV411)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 13 2021

    Western blot abreview for Anti-PU.1/Spi1 antibody [EPR3158Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse prefrontal cortex)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12% BisTris)
    Loading amount
    20 µg
    Specification
    Mouse prefrontal cortex
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 14 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-PU.1/Spi1 antibody [EPR3158Y]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Sample
    Human Tissue sections (Lymph node)
    Specification
    Lymph node
    Permeabilization
    No
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 15 2013

    Question

    Hi there,

    A customer would like to know if they are eligible for a testing discount for;

    ii. Products catalogue number: ab76543

    iii. Which application the customer would like to test: IHC-fr

    iv. Which species: Human

    v. Their sample type: lymph node, skin and liver tissues.

    Thanks for your help!

    Kind regards,

    Read More
    Answer

    DISCOUNT CODE: 42636
    Expiration date:15-Mar-13

    I am very pleased to hear a customer would like to accept our offer and test ab76543 in IHC-Fr. To redeem this offer, an Abreview needs to be submitted for IHC-Fr on human samples and this code put in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com