重组Anti-PTPRR/PCPTP1抗体[EPR13188] (ab180134)
![Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)](https://www.abcam.cn/ps/products/180/ab180134/Images/ab180134-210058-ab180134-wb.jpg "Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13188] to PTPRR/PCPTP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PTPRR/PCPTP1抗体[EPR13188]
参阅全部 PTPRR/PCPTP1 一抗 -
描述
兔单克隆抗体[EPR13188] to PTPRR/PCPTP1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PCPTP1/PTPRQ aa 1-100. The exact sequence is proprietary.
Database link: Q15256 -
阳性对照
- Human cerebellum, mouse and rat brain lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13188 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180134于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 74 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 74 kDa. |
靶标
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功能
Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. -
组织特异性
Expressed in brain, placenta, small intestine, stomach, uterus and weakly in the prostate. Isoform alpha has been observed only in the brain. Isoform gamma is expressed in brain, placenta and uterus. Isoform delta is expressed in brain, kidney, placenta, prostate, small intestine and uterus. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 7 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
细胞定位
Cell membrane and Cytoplasm > perinuclear region. Locates to the perinuclear areas within the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5801 Human
- Entrez Gene: 19279 Mouse
- Entrez Gene: 94202 Rat
- Omim: 602853 Human
- SwissProt: Q15256 Human
- SwissProt: Q62132 Mouse
- SwissProt: O08617 Rat
- Unigene: 506076 Human
see all -
别名
- Ch-1PTPase antibody
- Ch1 PTPase antibody
- DKFZp781C1038 antibody
see all
图片
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Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)All lanes : Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134) at 1/5000 dilution
Lane 1 : Human cerebellum lysate at 20 µg
Lane 2 : Mouse brain lysate at 20 µg
Lane 3 : Rat brain lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 74 kDa -
![Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)](https://www.abcam.cn/ps/products/180/ab180134/Images/ab180134-487225-anti-pcptp1-ptprq-antibody-epr13188-western-blot-human.jpg)
Western blot - Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)All lanes : Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134) at 1/1000 dilution
Lane 1 : HEK293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate
Lane 3 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate
Lane 4 : Human cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM /TBSTHEK-293, HCT116 and SK-OV-3 are low expressors (PMID: 25592066, PMID: 20015382, PMID: 31653698).
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![Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)](https://www.abcam.cn/ps/products/180/ab180134/Images/ab180134-2-benefits-of-recombinant-antibodies.png)
Anti-PTPRR/PCPTP1 antibody [EPR13188] (ab180134)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab180134 被引用在 1 文献中.
- Chang Y et al. Parvimonas micra activates the Ras/ERK/c-Fos pathway by upregulating miR-218-5p to promote colorectal cancer progression. J Exp Clin Cancer Res 42:13 (2023). PubMed: 36627634