AB126788

重组Anti-PTPRE抗体[EPR6715]

Anti-PTPRE antibody [EPR6715]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal PTPRE antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 3 publications.

查看别名

Receptor-type tyrosine-protein phosphatase epsilon, Protein-tyrosine phosphatase epsilon, R-PTP-epsilon, PTPRE

3 Images

Flow Cytometry (Intracellular) - Anti-PTPRE antibody [EPR6715] (AB126788)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PTPRE antibody [EPR6715] (AB126788)

ab126788 at 1/10 dilution staining PTPRE in permeabilized HL60 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).

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Western blot - Anti-PTPRE antibody [EPR6715] (AB126788)

All lanes:

Western blot - Anti-PTPRE antibody [EPR6715] (ab126788) at 1/1000 dilution

Lane 1:

HL60 cell lysate, treated with TPA at 10 µg

Lane 2:

HL60 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

C2C12 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 81 kDa

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OI-RD Scanning - Anti-PTPRE antibody [EPR6715] (AB126788)

OI-RD Scanning

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OI-RD Scanning - Anti-PTPRE antibody [EPR6715] (AB126788)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-PTPRE antibody [EPR6715] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6715

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PTPRE also known as Protein Tyrosine Phosphatase Receptor Type E or PTPε acts as a tyrosine phosphatase that removes phosphate groups from specific tyrosine residues on proteins. This enzyme which has a molecular mass of approximately 83 kDa functions as a receptor-type phosphatase with roles in cell signaling. PTPRE is expressed across various tissues including high levels in the brain and testis suggesting diverse functional roles in different cellular contexts.

Biological function summary

Protein Tyrosine Phosphatase Receptor Type E regulates cellular processes such as growth differentiation and motility by modulating the phosphorylation state of key signaling components. PTPRE is not known as part of a specific protein complex but interacts with various proteins through its enzymatic activity. It maintains balance in cellular signal transduction processes by counterbalancing kinases which add phosphate groups. This regulatory role indicates its involvement in many critical cellular activities and responses.

Pathways

Protein Tyrosine Phosphatase Receptor Type E influences the MAPK/ERK pathway which plays an important role in cell division and the JAK/STAT signaling pathway important for immune response. In these pathways PTPRE interacts and regulates proteins such as ERK and STAT respectively. By modulating phosphorylation PTPRE influences pathway signaling outcomes and cellular responses to external stimuli showing its integrative role in controlling signal transduction dynamics.

Protein Tyrosine Phosphatase Receptor Type E has been associated with cancer and autoimmune diseases. PTPRE's activity in dephosphorylating targets like ERK and STAT can impact cell proliferation and immune regulation linking it to tumor growth and immune dysregulation. In some cancers abnormal PTPRE activity interacts with other signaling proteins such as Src-family kinases influencing oncogenic processes. This association makes PTPRE a protein of interest for understanding and potentially targeting disease mechanisms.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function (By similarity).. Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake (By similarity).. Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).

See full target information PTPRE

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 30:459 PubMed40776904

2025

Knockdown of LINC00467 inhibits gastric cancer progression by modulating the sequestration of miR-141-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Ju,Yi Feng,Xiaojing Mu,Weitai He,Guifang He,Ben Tian,Duo Cai,Changchang Liu,Yu Song,Hao Chen,Shihai Liu

Frontiers in molecular neuroscience 14:688050 PubMed34630032

2021

Tandem Mass Tag-Based Quantitative Proteomic Analysis Reveals Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves.

Applications

Unspecified application

Species

Unspecified reactive species

Changci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Mingxiao Hou,Yunen Liu

Molecular biology of the cell 32:567-578 PubMed33566639

2021

Receptor-type protein tyrosine phosphatase alpha (PTPα) mediates MMP14 localization and facilitates triple-negative breast cancer cell invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa R Decotret,Brennan J Wadsworth,Ling Vicky Li,Chinten J Lim,Kevin L Bennewith,Catherine J Pallen

View all publications

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重组Anti-PTPRE抗体[EPR6715]