Anti-PTP1B (phospho S352)抗体[EP1839Y] (ab62330)
![Western blot - Anti-PTP1B (phospho S352) antibody [EP1839Y] (ab62330)](https://www.abcam.cn/ps/products/62/ab62330/Images/ab62330-75632-anti-ptp1b-phospho-s352-antibody-ep1839y-western-blot.jpg "Western blot - Anti-PTP1B (phospho S352) antibody [EP1839Y] (ab62330)")
Key features and details
- Rabbit monoclonal [EP1839Y] to PTP1B (phospho S352)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-PTP1B (phospho S352)抗体[EP1839Y]
参阅全部 PTP1B 一抗 -
描述
兔单克隆抗体[EP1839Y] to PTP1B (phospho S352) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cell lysate.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP1839Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62330于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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| 说明 |
|---|
|
WB
1/2500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
靶标
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功能
May play an important role in CKII- and p60c-src-induced signal transduction cascades. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
翻译后修饰
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5770 Human
- Omim: 176885 Human
- SwissProt: P18031 Human
- Unigene: 417549 Human
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别名
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
see all
图片
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Western blot - Anti-PTP1B (phospho S352) antibody [EP1839Y] (ab62330)All lanes : Anti-PTP1B (phospho S352) antibody [EP1839Y] (ab62330) at 1/5000 dilution
Lane 1 : Hela cell lysates (untreated)
Lane 2 : Hela cell lysates (treated with Calyculin A)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab62330 尚未被引用在任何文献中。