Anti-PTP1B抗体[OTI1D10] (ab124375)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)](https://www.abcam.cn/ps/products/124/ab124375/Images/ab124375-138009-anti-ptp1b-antibody-oti1d10-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)")
Key features and details
- Mouse monoclonal [OTI1D10] to PTP1B
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Rat, Dog, Human, African green monkey
- Isotype: IgG2a
概述
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产品名称
Anti-PTP1B抗体[OTI1D10]
参阅全部 PTP1B 一抗 -
描述
小鼠单克隆抗体[OTI1D10] to PTP1B -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Dog, Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human PTP1B.
Database link: NP_002818 -
阳性对照
- WB: PTP1B-transfected HEK293T, HepG2, HeLa, COS7, Jurkat, MDCK, PC12, and MCF7 cell lysates IHC-P: Human colon, Human lymph node and Human lymphoma tissuesICC/IF: COS7 cells transiently transfected by PTP1B. Flow Cyt (Intra): PTP1B-transfected HEK293T cells.
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常规说明
The clone number has been updated from 1D10 to OTI1D10, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI1D10 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124375于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
|
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| IHC-P |
1/150.
|
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| ICC/IF |
1/100.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa. |
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IHC-P
1/150. |
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ICC/IF
1/100. |
靶标
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功能
May play an important role in CKII- and p60c-src-induced signal transduction cascades. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
翻译后修饰
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5770 Human
- Entrez Gene: 24697 Rat
- Omim: 176885 Human
- SwissProt: P18031 Human
- SwissProt: P20417 Rat
- Unigene: 417549 Human
- Unigene: 11317 Rat
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别名
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)Staining of PTP1B in Paraffin-embedded Human colon tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.
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![Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)](https://www.abcam.cn/ps/products/124/ab124375/Images/ab124375-314675-anti-ptp1b-antibody-oti1d10-western-blot.jpg)
Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)All lanes : Anti-PTP1B antibody [OTI1D10] (ab124375)
Lane 1 : Wild-type HeLa cells
Lane 2 : PTPB1 knockout HeLa cells
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa -
![Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)](https://www.abcam.cn/ps/products/124/ab124375/Images/ab124375-138011-anti-ptp1b-antibody-oti1d10-western-blot.jpg)
Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)All lanes : Anti-PTP1B antibody [OTI1D10] (ab124375) at 1/500 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PTP1B
Lysates/proteins at 5 µg per lane.
Predicted band size: 50 kDa -
![Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)](https://www.abcam.cn/ps/products/124/ab124375/Images/ab124375-138010-anti-ptp1b-antibody-oti1d10-western-blot.jpg)
Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)All lanes : Anti-PTP1B antibody [OTI1D10] (ab124375) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)](https://www.abcam.cn/ps/products/124/ab124375/Images/ab124375-138008-anti-ptp1b-antibody-oti1d10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)Staining of PTP1B in Paraffin-embedded Human lymph node tissue by Immunohistochemistry using ab124375 at a dilution of 1/150. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)](https://www.abcam.cn/ps/products/124/ab124375/Images/ab124375-138007-anti-ptp1b-antibody-oti1d10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (ab124375)Staining of PTP1B in Paraffin-embedded Human lymphoma tissue by Immunohistochemistry using ab124375 at a dilution of 1/150. -
![Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [OTI1D10] (ab124375)](https://www.abcam.cn/ps/products/124/ab124375/Images/ab124375-138006-anti-ptp1b-antibody-oti1d10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [OTI1D10] (ab124375)Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTP1B using ab124375 at a dilution of 1/100. -
![Flow Cytometry (Intracellular) - Anti-PTP1B antibody [OTI1D10] (ab124375)](https://www.abcam.cn/ps/products/124/ab124375/Images/ab124375-138005-anti-ptp1b-antibody-oti1d10-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PTP1B antibody [OTI1D10] (ab124375)HEK293T cells transfected with either PTP1B overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab124375 at a dilution of 1/100, and then analyzed by Flow Cytometry (Intracellular).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab124375 被引用在 3 文献中.
- Morris R et al. Structure guided studies of the interaction between PTP1B and JAK. Commun Biol 6:641 (2023). PubMed: 37316570
- Joy-Immediato M et al. Junctional ER Organization Affects Mechanotransduction at Cadherin-Mediated Adhesions. Front Cell Dev Biol 9:669086 (2021). PubMed: 34222239
- Gabay O et al. Sirtuin 1 enzymatic activity is required for cartilage homeostasis in vivo in a mouse model. Arthritis Rheum 65:159-66 (2013). IHC ; Mouse . PubMed: 23124828