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AB124375

Anti-PTP1B抗体[OTI1D10]

Anti-PTP1B antibody [OTI1D10]

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(3 Publications)

Mouse Monoclonal PTP1B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with African green monkey, Dog, Rat, Transfected cell lysate - Human, Human, Recombinant fragment - Human, Transfected cell line - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PTPN1.

查看别名

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (AB124375)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (AB124375)

Staining of PTP1B in Paraffin-embedded Human lymphoma tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (AB124375)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (AB124375)

Staining of PTP1B in Paraffin-embedded Human colon tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.

Flow Cytometry (Intracellular) - Anti-PTP1B antibody [OTI1D10] (AB124375)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PTP1B antibody [OTI1D10] (AB124375)

HEK293T cells transfected with either PTP1B overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab124375 at a dilution of 1/100, and then analyzed by Flow Cytometry (Intracellular).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (AB124375)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B antibody [OTI1D10] (AB124375)

Staining of PTP1B in Paraffin-embedded Human lymph node tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [OTI1D10] (AB124375)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [OTI1D10] (AB124375)

Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTP1B using ab124375 at a dilution of 1/100.

Western blot - Anti-PTP1B antibody [OTI1D10] (AB124375)
  • WB

Supplier Data

Western blot - Anti-PTP1B antibody [OTI1D10] (AB124375)

All lanes:

Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375) at 1/500 dilution

Lane 1:

HEK293T cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK293T cell lysate transfected with pCMV6-ENTRY PTP1B at 5 µg

Predicted band size: 50 kDa

false

Western blot - Anti-PTP1B antibody [OTI1D10] (AB124375)
  • WB

Lab

Western blot - Anti-PTP1B antibody [OTI1D10] (AB124375)

All lanes:

Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375)

Lane 1:

Wild-type HeLa cells at 10 µg

Lane 2:

PTPB1 knockout HeLa cells at 10 µg

Predicted band size: 50 kDa

false

Western blot - Anti-PTP1B antibody [OTI1D10] (AB124375)
  • WB

Supplier Data

Western blot - Anti-PTP1B antibody [OTI1D10] (AB124375)

All lanes:

Western blot - Anti-PTP1B antibody [OTI1D10] (ab124375) at 1/500 dilution

Lane 1:

HepG2 cell extract at 35 µg

Lane 2:

HeLa cell extract at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 cell extract at 35 µg

Lane 5:

COS7 cell extract at 35 µg

Lane 6:

Jurkat cell extract at 35 µg

Lane 7:

MDCK cell extract at 35 µg

Lane 8:

PC12 cell extract at 35 µg

Lane 9:

MCF7 cell extract at 35 µg

Predicted band size: 50 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

OTI1D10

亚型

IgG2a

不含载体蛋白

No

反应种属

Rat, Dog, Human, African green monkey

应用

WB, ICC/IF, IHC-P, Flow Cyt (Intra)

applications

免疫原

Recombinant Full Length Protein corresponding to Human PTPN1.

P18031

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

The clone number has been updated from 1D10 to OTI1D10, both clone numbers name the same clone.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
纯化说明
Purified from cell culture supernatant by affinity chromatography
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
See full target information PTPN1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:641 PubMed37316570

2023

Structure guided studies of the interaction between PTP1B and JAK.

Applications

Unspecified application

Species

Unspecified reactive species

Rhiannon Morris,Narelle Keating,Cyrus Tan,Hao Chen,Artem Laktyushin,Tamanna Saiyed,Nicholas P D Liau,Nicos A Nicola,Tony Tiganis,Nadia J Kershaw,Jeffrey J Babon

Frontiers in cell and developmental biology 9:669086 PubMed34222239

2021

Junctional ER Organization Affects Mechanotransduction at Cadherin-Mediated Adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle Joy-Immediato,Manuel J Ramirez,Mauricio Cerda,Yusuke Toyama,Andrea Ravasio,Pakorn Kanchanawong,Cristina Bertocchi

Arthritis and rheumatism 65:159-66 PubMed23124828

2012

Sirtuin 1 enzymatic activity is required for cartilage homeostasis in vivo in a mouse model.

Applications

IHC

Species

Mouse

Odile Gabay,Christelle Sanchez,Mona Dvir-Ginzberg,Viktoria Gagarina,Kristien J Zaal,Yingjie Song,Xiao Hong He,Michael W McBurney
View all publications
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搭配使用产品

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