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重组RabMAb

重组Anti-PTP1B + TCPTP抗体[EPR6712] (ab129070)

Submit a review Submit a question References (1)
Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
  • Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
  • Flow Cytometry (Intracellular) - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
  • Immunoprecipitation - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
  • Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6712] to PTP1B + TCPTP
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-PTP1B + TCPTP抗体[EPR6712]

  • 描述

    兔单克隆抗体[EPR6712] to PTP1B + TCPTP

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IPmore details
    不适用于: ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HL60, NIH 3T3, MOLT4 and HepG2 whole cell lysate (ab7900); Mouse placenta and thymus lysates.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab129070于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/200.
WB
1/1000 - 1/10000. Detects a band of approximately 45-48 kDa (predicted molecular weight: 48 kDa).
IP
1/10 - 1/100.
说明
Flow Cyt (Intra)
1/200.
WB
1/1000 - 1/10000. Detects a band of approximately 45-48 kDa (predicted molecular weight: 48 kDa).
IP
1/10 - 1/100.
应用说明
Is unsuitable for ICC/IF or IHC-P.

靶标

图片

  • Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
    Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
    Lanes 1-2 : Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070) at 1/1000 dilution
    Lanes 3-4 : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution

    Lanes 1 & 3 : N-GST tagged full length recombinant human TCPTP (PTPN2) protein 10ng
    Lanes 2 & 4 : N-GST tagged full length recombinant human PTP1B (PTPN1) protein 10ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa
    Observed band size: 68-75 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    N-GST tagged full length recombinant human TCPTP protein (Cat#: ab268903)

    N-GST tagged Full length Recombinant human PTP1B protein (Cat#: ab268897)

  • Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
    Western blot - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
    All lanes : Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070) at 1/1000 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : NIH/3T3 cell lysate
    Lane 3 : Mouse placenta tissue lysate
    Lane 4 : Mouse thymus tissue lysate
    Lane 5 : MOLT4 cell lysate
    Lane 6 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 48 kDa
    Observed band size: 45-48 kDa why is the actual band size different from the predicted?

  • Flow Cytometry (Intracellular) - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
    Flow Cytometry (Intracellular) - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)

    Intracellular flow cytometric analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) cell linefixed with 4% paraformaldehyde and permeabilized with 90% methanollabeling TCPTP with ab129070 at 1/200 dilution (red). This is compared with a Rabbit monoclonal IgG - Isotype control (ab172730) (black) and a unalbelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-rabbit IgG (Alexa Fluorr®488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
    Immunoprecipitation - Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)

    Lane 1 (input): MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate, 10μg
    Lane 2 (+): MOLT-4 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129070 in MOLT-4 whole cell lysate.

    Ab129070 Immunoprecipitating TCPTP in MOLT-4 whole cell lysate. Capture antibody was used at a 1:20 dilution (0.6μg in 0.35mg lysates). For western blotting, primary antibody used was ab129070 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: 1second.

  • Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)
    Anti-PTP1B + TCPTP antibody [EPR6712] (ab129070)

文献 (1)

发表研究结果有使用 ab129070？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab129070 被引用在 1 文献中.

  • Tun MT  et al. Macromolecular crowding amplifies allosteric regulation of T-cell protein tyrosine phosphatase. J Biol Chem 298:102655 (2022). PubMed: 36328244

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