Anti-PTN抗体- C-terminal (ab223674)
Key features and details
- Goat polyclonal to PTN - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PTN抗体- C-terminal
参阅全部 PTN 一抗 -
描述
山羊多克隆抗体to PTN - C-terminal -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chicken, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human PTN aa 150 to the C-terminus (C terminal) (Cysteine residue). NP_002816.1.
Database link: P21246 -
阳性对照
- WB: Mouse fetal brain lysate. IHC-P: Human prostate tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab223674 purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223674于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18.9 kDa).
1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18.9 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Heparin binding mitogenic protein. Has neurite extension activity. -
组织特异性
Osteoblast and brain. -
序列相似性
Belongs to the pleiotrophin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 280904 Cow
- Entrez Gene: 5764 Human
- Entrez Gene: 19242 Mouse
- Entrez Gene: 397609 Pig
- Entrez Gene: 24924 Rat
- Omim: 162095 Human
- SwissProt: P32760 Chicken
- SwissProt: P21782 Cow
see all -
别名
- HARP antibody
- HB-GAM antibody
- HBBM antibody
see all
图片
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Western blot - Anti-PTN antibody - C-terminal (ab223674)Anti-PTN antibody - C-terminal (ab223674) at 0.1 µg/ml + mouse fetal brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 18.9 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (1)
ab223674 被引用在 1 文献中.
- Liu S et al. Knockdown of pleiotrophin increases the risk of preeclampsia following vitrified-thawed embryo transfer. Int J Oncol 53:1847-1856 (2018). PubMed: 30226583
