Anti-PTGIS/PGIS抗体(ab23668)
Key features and details
- Rabbit polyclonal to PTGIS/PGIS
- Suitable for: WB, ICC/IF, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Sheep, Cow, Human
- Isotype: IgG
概述
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产品名称
Anti-PTGIS/PGIS抗体
参阅全部 PTGIS/PGIS 一抗 -
描述
兔多克隆抗体to PTGIS/PGIS -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Sheep, Cow, Human -
免疫原
Synthetic peptide corresponding to Mouse PTGIS/PGIS aa 450-550.
Database link: O35074 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading... -
纯化说明
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23668于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration.
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2). -
组织特异性
Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 282021 Cow
- Entrez Gene: 5740 Human
- Entrez Gene: 19223 Mouse
- Entrez Gene: 25527 Rat
- Omim: 601699 Human
- SwissProt: Q29626 Cow
- SwissProt: Q16647 Human
- SwissProt: O35074 Mouse
see all -
别名
- CYP8 antibody
- CYP8A1 antibody
- Cytochrome P450, family 8, subfamily A, polypeptide 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTGIS/PGIS antibody (ab23668)
ab23668 (4µg/ml) staining PTGIS/PGIS in human adrenal gland using an automated system (DAKO Autostainer Plus). Using this protocol there is apical cytoplasmic/ endoplasmic reticulum staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
数据表及文件
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Datasheet download
文献 (16)
ab23668 被引用在 16 文献中.
- Zhou T et al. Key Molecules in Bladder Cancer Affect Patient Prognosis and Immunotherapy Efficacy: Further Exploration for CNTN1 and EMP1. JCO Precis Oncol 7:e2200630 (2023). PubMed: 37437228
- Aakerøy L et al. Identification of exercise-regulated genes in mice exposed to cigarette smoke. Physiol Rep 10:e15505 (2022). PubMed: 36324300
- Bae SJ et al. Identification and analysis of novel endometriosis biomarkers via integrative bioinformatics. Front Endocrinol (Lausanne) 13:942368 (2022). PubMed: 36339397
- Su C et al. Single-Cell RNA Sequencing in Multiple Pathologic Types of Renal Cell Carcinoma Revealed Novel Potential Tumor-Specific Markers. Front Oncol 11:719564 (2021). PubMed: 34722263
- Pan XY et al. Alternative activation of macrophages by prostacyclin synthase ameliorates alcohol induced liver injury. Lab Invest 101:1210-1224 (2021). PubMed: 34112940
