AB109407

Anti-PTCHD1抗体

Name: PTCHD1抗体 (ab109407)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109407
Price: 4606 CNY
Availability: InStock

Anti-PTCHD1 antibody

(1 Review)

(3 Publications)

Goat Polyclonal PTCHD1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PTCHD1 aa 250-300.

查看别名

Patched domain-containing protein 1, PTCHD1

1 Images

Unknown

Western blot - Anti-PTCHD1 antibody (AB109407)

An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide.

All lanes:

Western blot - Anti-PTCHD1 antibody (ab109407) at 0.5 µg/mL

All lanes:

Human Brain Cerebellum lysate in RIPA buffer at 35 µg

Predicted band size: 101 kDa

true

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human PTCHD1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q96NR3

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab109407 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PTCHD1 also known as Patched domain-containing protein 1 is a protein with a mass of approximately 139 kDa. This protein functions as a receptor that interacts with and regulates the Hedgehog signaling pathway. PTCHD1 is widely expressed in the human brain particularly in both neuronal and glial cells. This expression pattern suggests a significant role in neural development and function. The protein structure includes a large extracellular domain allowing it to bind with various ligands associated with cell signaling processes.

Biological function summary

PTCHD1 plays an important role in modulating the Hedgehog signaling pathway which is essential for cell differentiation and growth. This protein is thought to work as a part of a complex involving the regulation of cell membrane-associated signaling proteins. Its presence ensures correct signaling dynamics influencing cellular processes such as proliferation and tissue patterning during development. PTCHD1's function highlights its potential impact on the proper organization and operation of the neural environments.

Pathways

PTCHD1 is significantly involved in the Hedgehog signaling and its regulation. This pathway is important for embryonic development and various postnatal processes. PTCHD1 interacts with other proteins in this pathway such as Smoothened (SMO) and the GLI family of transcription factors coordinating the transmission of signals that govern cellular fate decisions. Through these interactions PTCHD1 influences processes from growth and differentiation to apoptosis.

Alterations in PTCHD1 expression or function relate to neurodevelopmental disorders such as autism spectrum disorders (ASD) and intellectual disabilities. Studies have shown that PTCHD1 mutations can disrupt normal Hedgehog signaling influencing brain development and function. Additionally PTCHD1's interaction with other proteins like SHANK3 highlights its significance in maintaining synaptic integrity and function further associating it with ASD and related conditions. Understanding these connections can aid in developing targeted therapies for these complex disorders.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Required for the development and function of the thalamic reticular nucleus (TRN), a part of the thalamus that is critical for thalamocortical transmission, generation of sleep rhythms, sensorimotor processing and attention. Can bind cholesterol in vitro (PubMed : 36769003).

See full target information PTCHD1

style

left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 30:677-688.e5 PubMed37019105

2023

Generation of ventralized human thalamic organoids with thalamic reticular nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Ferdi Ridvan Kiral,Bilal Cakir,Yoshiaki Tanaka,Jonghun Kim,Woo Sub Yang,Fabien Wehbe,Young-Jin Kang,Mei Zhong,Gizem Sancer,Sang-Hun Lee,Yangfei Xiang,In-Hyun Park

Stem cells international 2023:1662182 PubMed39280589

2023

Hypoxia-Elicited Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Alleviate Myocardial Infarction by Promoting Angiogenesis through the miR-214/Sufu Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lianbo Shao,Yihuan Chen,Jingjing Li,Jingfan Chao,Ziying Yang,Yinglong Ding,Han Shen,Yueqiu Chen,Zhenya Shen

Nature communications 11:2588 PubMed32444594

2020

KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Lipinski,Rafael Muñoz-Viana,Beatriz Del Blanco,Angel Marquez-Galera,Juan Medrano-Relinque,José M Caramés,Andrzej A Szczepankiewicz,Jordi Fernandez-Albert,Carmen M Navarrón,Roman Olivares,Grzegorz M Wilczyński,Santiago Canals,Jose P Lopez-Atalaya,Angel Barco

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTCHD1抗体

Anti-PTCHD1 antibody

Western blot - Anti-PTCHD1 antibody (AB109407)