重组Anti-PSPH抗体[001] - BSA and Azide free

• ICC

Supplier Data

Immunocytochemistry - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)

Immunofluorescence staining of Human PSPH in A549 (human lung carcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276045 at 1/60 dilution at 4℃ overnight. Then cells were stained with the AlexaFluor^® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

• IP

Unknown

Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)

PSPH was immunoprecipitated using from :
Lane A : 0.5 mg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole Cell Lysate
Lane B : 0.5 mg HepG2 (human liver hepatocellular carcinoma cell line) whole Cell Lysate

0.5 μL ab276045 and 15 μl of 50 % Protein G agarose.
Primary antibody : ab276045, at 1/1000 dilution
Secondary antibody : Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution

Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-PSPH antibody [001] - BSA and Azide free (ab276045)

Predicted band size: 25 kDa

false

• WB

Unknown

Western blot - Anti-PSPH antibody [001] - BSA and Azide free (AB276045)

Developed using the Odyssey technique.

Performed under reducing conditions.

All lanes:

Western blot - Anti-PSPH antibody [001] - BSA and Azide free (ab276045) at 1/500 dilution

Lane 1:

MCF7 whole cell lysate at 30 µg

Lane 2:

K562 whole cell lysate at 30 µg

Lane 3:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 25 kDa

false