重组Anti-PspCas13b抗体[12F2-A10] (ab273440)
![Western blot - Anti-PspCas13b antibody [12F2-A10] (ab273440)](https://www.abcam.cn/ps/products/273/ab273440/Images/ab273440-455051-anti-pspcas13b-antibody-12f2a10-western-blot-hek293t.jpg "Western blot - Anti-PspCas13b antibody [12F2-A10] (ab273440)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [12F2-A10] to PspCas13b
- Suitable for: WB
Related conjugates and formulations
概述
-
产品名称
Anti-PspCas13b抗体[12F2-A10]
参阅全部 PspCas13b 一抗 -
描述
小鼠单克隆抗体[12F2-A10] to PspCas13b -
宿主
Mouse -
特异性
Reacts with Prevotella sp. P5, 125
-
经测试应用
适用于: WBmore details -
种属反应性
与反应: Prevotella pectinovora
不与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HEK-293T transfected with PspCas13b expression vector containing a myc-His-tag®, whole cell lysate.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
12F2-A10 -
同种型
IgG2a
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273440于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000.
|
| 说明 |
|---|
|
WB
1/1000. |
图片
-
Western blot - Anti-PspCas13b antibody [12F2-A10] (ab273440)All lanes : Anti-PspCas13b antibody [12F2-A10] (ab273440) at 1/5000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with PspCas13b expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/20000 dilution
Exposure time: 10 secondsLysates should be made freshly and used in WB immediately to minimize protein degradation.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-PspCas13b antibody [12F2-A10] (ab273440)](https://www.abcam.cn/ps/products/273/ab273440/Images/ab273440-455052-anti-pspcas13b-antibody-12f2a10-western-blot-hela.jpg)
Western blot - Anti-PspCas13b antibody [12F2-A10] (ab273440)All lanes : Anti-PspCas13b antibody [12F2-A10] (ab273440) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/20000 dilution
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-PspCas13b antibody [12F2-A10] (ab273440)](https://www.abcam.cn/ps/products/273/ab273440/Images/ab273440-455154-benefitsofrecombinantantibodies.jpg)
Anti-PspCas13b antibody [12F2-A10] (ab273440)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab273440 尚未被引用在任何文献中。