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重组RabMAb

重组Anti-Psoriasin抗体[EPR23482-38] (ab275026)

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Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
  • Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
  • Flow Cytometry (Intracellular) - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
  • Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
  • Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
  • Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
  • Anti-Psoriasin antibody [EPR23482-38] (ab275026)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23482-38] to Psoriasin
  • Suitable for: IP, Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Psoriasin抗体[EPR23482-38]
    参阅全部 Psoriasin 一抗

  • 描述

    兔单克隆抗体[EPR23482-38] to Psoriasin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IP, Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: MDA-MB-468 whole cell lysate; Human tonsil tissue lysate; His-tagged human recombinant Psoriasin (Protein S100-A7) protein; His-tagged human recombinant S100-A7A protein. IHC-P: Human skin and tonsil tissue. ICC/IF: MDA-MB-468 cells. Flow Cyt (intra): MDA-MB-468 cells. IP: MDA-MB-468 whole cell lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab275026于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
1/30.
Flow Cyt (Intra)
1/500.
ICC/IF
1/250.
WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 11 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
IP
1/30.
Flow Cyt (Intra)
1/500.
ICC/IF
1/250.
WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 11 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 组织特异性

    Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients.

  • 序列相似性

    Belongs to the S-101 family.
    Contains 2 EF-hand domains.

  • 细胞定位

    Cytoplasm. Secreted. Secreted by a non-classical secretory pathway.
  • Information by UniProt

  • 数据库链接

    • 别名

      • HID 5 antibody
      • Protein S100 A7 antibody
      • Protein S100-A7 antibody
      • PSOR 1 antibody
      • PSOR1 antibody
      • Psoriasin 1 antibody
      • Psoriasin antibody
      • Psoriasin1 antibody
      • S100 Calcium binding protein A7 antibody
      • S100 calcium-binding protein A7 antibody
      • S100A7 antibody
      • S100A7c antibody
      • S10A7_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Anti-Psoriasin antibody [EPR23482-38] (ab275026) at 1/1000 dilution + Human tonsil tissue lysate at 20 µg

      Secondary
      VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

      Predicted band size: 11 kDa
      Observed band size: 12 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 81 seconds.

      The expression profile observed is consistent with what has been described in the literature (PMID: 20596736, 12855640, 26055798).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] (ab275026)

      Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Psoriasin with ab275026 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human skin (PMID: 18223693). The section was incubated with ab275026 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [EPR23482-38] (ab275026)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-468 cells labelling Psoriasin with ab275026 at 1/250 (2.132 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MDA-MB-468 cells.

      Negative control: MDA-MB-231 (PMID: 15994944).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Flow Cytometry (Intracellular) - Anti-Psoriasin antibody [EPR23482-38] (ab275026)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MDA-MB-468 (Human breast adenocarcinoma epithelial cell) cells labelling Psoriasin with ab275026 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Immunoprecipitation - Anti-Psoriasin antibody [EPR23482-38] (ab275026)

      Psoriasin was immunoprecipitated from 0.35 mg MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate with ab275026 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275026 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: MDA-MB-468 whole cell lysate 10 µg.

      Lane 2: ab275026 IP in MDA-MB-468 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275026 in MDA-MB-468 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 76 seconds.

       

    • Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      All lanes : Anti-Psoriasin antibody [EPR23482-38] (ab275026) at 1/1000 dilution

      Lane 1 : His-tagged human recombinant Psoriasin (Protein S100-A7) protein, 500 ng
      Lane 2 : His-tagged human recombinant S100-A7A protein, 500 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 11 kDa
      Observed band size: 16 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 8 seconds.

      This antibody reacts with Psoriasin (Protein S100-A7) and Protein S100-A7A.
      Linking with 6x His tag leads to the shift of the bands .
      Both recombinant proteins were made in house and extracted from E.coli expressing Psoriasin (Protein S100-A7) or Protein S100-A7A.

       

    • Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Western blot - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      All lanes : Anti-Psoriasin antibody [EPR23482-38] (ab275026) at 1/1000 dilution

      Lane 1 : MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 11 kDa
      Observed band size: 12 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      The expression profile observed is consistent with what has been described in the literature (PMID: 20596736, 12855640, 26055798).

      Negative control: MDA-MB-231 (PMID: 12855640).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [EPR23482-38] (ab275026)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Psoriasin with ab275026 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab275026 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-Psoriasin antibody [EPR23482-38] (ab275026)
      Anti-Psoriasin antibody [EPR23482-38] (ab275026)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab275026？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab275026 尚未被引用在任何文献中。

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