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Anti-Psoriasin抗体[47C1068] (ab13680)

Reviews (2)Q&A (5)References (25)
Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [47C1068] (ab13680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [47C1068] (ab13680)
  • Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)
  • Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [47C1068] (ab13680)

Key features and details

  • Mouse monoclonal [47C1068] to Psoriasin
  • Suitable for: IP, ICC/IF, Flow Cyt, IHC-Fr, Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Psoriasin抗体[47C1068]
    参阅全部 Psoriasin 一抗

  • 描述

    小鼠单克隆抗体[47C1068] to Psoriasin

  • 宿主

    Mouse

  • 经测试应用

    适用于: IP, ICC/IF, Flow Cyt, IHC-Fr, Flow Cyt (Intra), WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein corresponding to Human Psoriasin.
    Database link: P31151

  • 阳性对照

    • Confluent MCF10A cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab13680于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt (1)
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr (1)
Use at an assay dependent concentration.

(PMID 18606705).
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 12.1 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
IP
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr
Use at an assay dependent concentration.

(PMID 18606705).
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 12.1 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 组织特异性

    Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients.

  • 序列相似性

    Belongs to the S-101 family.
    Contains 2 EF-hand domains.

  • 细胞定位

    Cytoplasm. Secreted. Secreted by a non-classical secretory pathway.
  • Information by UniProt

  • 数据库链接

    • 别名

      • HID 5 antibody
      • Protein S100 A7 antibody
      • Protein S100-A7 antibody
      • PSOR 1 antibody
      • PSOR1 antibody
      • Psoriasin 1 antibody
      • Psoriasin antibody
      • Psoriasin1 antibody
      • S100 Calcium binding protein A7 antibody
      • S100 calcium-binding protein A7 antibody
      • S100A7 antibody
      • S100A7c antibody
      • S10A7_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)
      Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)
      All lanes : Anti-Psoriasin antibody [47C1068] (ab13680) at 1 µg/ml

      Lane 1 : MCF10A cell lysate
      Lane 2 : MCF7 cell lysate

      Predicted band size: 12.1 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [47C1068] (ab13680)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [47C1068] (ab13680)

      Immunohistochemistry analysis of human esophageal squamous cell carcinoma (SCC) staining psoriasin with ab13680 in 5 µg/mL concentration.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [47C1068] (ab13680)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [47C1068] (ab13680)

      Immunohistochemistry analysis of formalin-fixed paraffin-embedded human skin tissue after heat-induced antigen retrieval, staining psoriasin with ab13680 in 10 µg/mL concentration.

    • Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)
      Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)

      Western blot analysis of Psoriasin in confluent MCF10A cells using ab13680 at 1 µg/ml.

      Western blot analysis of Psoriasin in confluent MCF10A cells using ab13680 at 1 µg/ml.
    • Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [47C1068] (ab13680)
      Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [47C1068] (ab13680)
      ICC/IF image of ab13680 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13680, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    文献 (25)

    发表研究结果有使用 ab13680？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab13680 被引用在 25 文献中.

    • Jung KL  et al. Single-cell analysis of Kaposi's sarcoma-associated herpesvirus infection in three-dimensional air-liquid interface culture model. PLoS Pathog 18:e1010775 (2022). PubMed: 35976902
    • Luo M  et al. Weighted gene coexpression network and experimental analyses identify lncRNA SPRR2C as a regulator of the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis. Cell Death Dis 12:86 (2021). PubMed: 33452236
    • Nguyen T  et al. Effective inhibition of Th17/Th22 pathway in 2D and 3D human models of psoriasis by Celastrol enriched plant cell culture extract. J Eur Acad Dermatol Venereol 34 Suppl 6:3-9 (2020). PubMed: 32783265
    • Shi ZR  et al. Decrease of galectin-3 in keratinocytes: A potential diagnostic marker and a critical contributor to the pathogenesis of psoriasis. J Autoimmun 89:30-40 (2018). WB ; Human . PubMed: 29167025
    • Rangaraj A  et al. Molecular and cellular impact of Psoriasin (S100A7) on the healing of human wounds. Exp Ther Med 13:2151-2160 (2017). PubMed: 28565822
    View all Publications for this product

    客户评价及客户问答

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Psoriasin antibody [47C1068]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% Trititon X-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 17 2012

    Flow Cytometry abreview for Anti-Psoriasin antibody [47C1068]

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (corneal epithelial cells)
    Specification
    corneal epithelial cells
    Preparation
    Cell harvesting/tissue preparation method: Human SV40-transformed immortalised corneal epithelial cell line
    Sample buffer: Optimized culture medium containing Epilife (Cascade Biologics, Mansfield, U.K.)
    Fixation
    Formaldehyde
    Permeabilization
    Yes - 0.5% Tween 20
    Gating Strategy
    around 90% of middle area of cell population
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 30 2010

    Question

    vielen Dank für die schnelle Antwort. Gerne möchte ich den Antikörper 13680 anti Psoriasin in Maus testen. Bitte schicken Sie mir den Rabattcode zu.

    Read More
    Answer

    Es freut mich zu hören, dass Sie unser Angebot unseren ab13680 Anti-Psoriasin [47C1068] Antikörper in der Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer 123456789, deckt den Wert von ab13680 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

    Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Maus eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

    Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

    Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

    Read More

    Question

    will it work in ms and rat?

    Read More
    Answer

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Unseres Wissens nach wurde ab13680 Anti-Psoriasin antibody [47C1068] bisher noch nicht in Maus getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab13680 abdecken, und gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab13680 in Maus testen möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper in Maus.

    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörper zu dem gleichen Preis wie ab13680 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab13680 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More

    Question

    Hi there, I am planning to make immunoaffinity column with this anti-psoriasin antibody. Do you have any data about making Immunoaffinity column (antibody affinity chromatography) ? Which antigenic determinant (epitope) of psoriasin is recognized by this monoclonal antibody ? Epitope= the part of an antigen that is recognized by the antibody.

    Read More
    Answer

    Thank you for contacting Abcam. The epitope region of ab13680 has not been mapped and so I cannot inform you as to where the antibody actually binds. As for making an immunoaffinity column

    Read More

    Question

    1. Can you please detail this; are you observing differences between different batches or variability as the result of repeats from the same experiment using the same antibody batch? Can you tell me in what was was the result inconsistent. Was low signal observed or staining that was inconsistent? I am observing differences between different batches. Using the same dilution, the intensity of my results are noticeably different from the first batch of antibody I received. I experienced low signal when using the same dilution of 1:100 between the different batches. 2. I would also appreciate it if you could provide me with details of the antigen retrieval approach that you are using; duration, approach etc. The slides were boiled in target retrieval solution byDakocytomation. The solution which is 10x is diluted to 1x and boiled in a pressure cooker for 10mins at 120 celsius. 3. Have you been successful using this approach with other antibodies? YES, WITH THE FIRST BATCH OF ANTIBODY, MY RESULTS WERE GOOD. 4. Are the samples that you are using human? HUMAN SAMPLES

    Read More
    Answer

    Thank you for getting back in touch with and for providing me with further details. The difficulties that you have been having are clearly lot related problems. I would like to offer you a free of charge replacement vial of a different lot. However, unfortunately the lot number that you received on order 152018 (PO number: 0001862788) was the same as the lot currently in stock. I would therefore like to offer you a credit note to the value of one vial of this antibody. If this is acceptable please email me and I will request that our accounts department processes your order details. I look forward to hearing from you.

    Read More

    Question

    BATCH NUMBER 47C1068 ORDER NUMBER 00018627 DESCRIPTION OF THE PROBLEM low signal. not consistent result as first order of the antibody. SAMPLE tumor tissues PRIMARY ANTIBODY 1:100 dilution. tried 1:50 dilution but signal still not very strong. ran a pos. control with the rest of the slides. DETECTION METHOD fast red tablet in substrate buffer. POSITIVE AND NEGATIVE CONTROLS USED positive controls used ANTIBODY STORAGE CONDITIONS -20 celsius FIXATION OF SAMPLE parafin embeded tissues on slides BLOCKING CONDITIONS target solution from [another company] SECONDARY ANTIBODY [another company] kit using polymer labelled AP as secondary antibody. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No WHAT STEPS HAVE YOU ALTERED? no steps altered. just ordered a new antibody

    Read More
    Answer

    Thank you for your enquiry. I am sorry to hear that you have had variable results using this antibody. I have read your questionaire and I have a few comments. I am most interested in the variability that you have been observing. 1. Can you please detail this; are you observing differences between different batches or variability as the result of repeats from the same experiment using the same antibody batch? Can you tell me in what was was the result inconsistent. Was low signal observed or staining that was inconsistent? 2. I would also appreciate it if you could provide me with details of the antigen retrieval approach that you are using; duration, approach etc. 3. Have you been successful using this approach with other antibodies? 4. Are the samples that you are using human? I appreciate your patience and look forward to hearing from you.

    Read More

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