重组Anti-PSMD14抗体[EPR4257] (ab109123)
![Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)](https://www.abcam.cn/ps/products/109/ab109123/Images/ab109123-121715-anti-psmd14-antibody-epr4257-western-blot.jpg "Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4257] to PSMD14
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PSMD14抗体[EPR4257]
参阅全部 PSMD14 一抗 -
描述
兔单克隆抗体[EPR4257] to PSMD14 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4257 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109123于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C allow to cool for 10-20 min). |
|
| ICC/IF |
1/500 - 1/1000.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min). |
|
ICC/IF
1/500 - 1/1000. |
应用说明
Is unsuitable for Flow Cyt or IP.
靶标
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功能
Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear. -
组织特异性
Widely expressed. Highest levels in heart and skeletal muscle. -
序列相似性
Belongs to the peptidase M67A family. PSMD14 subfamily.
Contains 1 MPN (JAB/Mov34) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 10213 Human
- Entrez Gene: 59029 Mouse
- Entrez Gene: 311078 Rat
- Omim: 607173 Human
- SwissProt: O00487 Human
- SwissProt: O35593 Mouse
- Unigene: 567410 Human
- Unigene: 218198 Mouse
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别名
- 26S proteasome non-ATPase regulatory subunit 14 antibody
- 26S proteasome regulatory subunit rpn11 antibody
- 26S proteasome-associated PAD1 homolog 1 antibody
see all
图片
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Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution
Lane 1 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Namalwa (Burkitt's lymphoma cell line) whole cell lysates
Lane 5 : K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (ab109123)](https://www.abcam.cn/ps/products/109/ab109123/Images/ab109123-121714-anti-psmd14-antibody-epr4257-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (ab109123)ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (ab109123)](https://www.abcam.cn/ps/products/109/ab109123/Images/ab109123-121713-anti-psmd14-antibody-epr4257-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (ab109123)ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence. -
![Anti-PSMD14 antibody [EPR4257] (ab109123)](https://www.abcam.cn/ps/products/109/ab109123/Images/ab109123-4-benefits-of-recombinant-antibodies.png)
Anti-PSMD14 antibody [EPR4257] (ab109123)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab109123 被引用在 5 文献中.
- Bashore C et al. Targeted degradation via direct 26S proteasome recruitment. Nat Chem Biol 19:55-63 (2023). PubMed: 36577875
- Finelli R et al. In Silico Sperm Proteome Analysis to Investigate DNA Repair Mechanisms in Varicocele Patients. Front Endocrinol (Lausanne) 12:757592 (2021). PubMed: 34975746
- Sun T et al. Deubiquitinase PSMD14 promotes ovarian cancer progression by decreasing enzymatic activity of PKM2. Mol Oncol 15:3639-3658 (2021). PubMed: 34382324
- Lei J et al. The prognostic value of USP14 and PSMD14 expression in non-small cell lung cancer. Ann Transl Med 9:1019 (2021). PubMed: 34277819
- Sakai W et al. Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair. Sci Rep 10:19704 (2020). PubMed: 33184426