Anti-PSMD1抗体(ab2941)
Key features and details
- Rabbit polyclonal to PSMD1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-PSMD1抗体
参阅全部 PSMD1 一抗 -
描述
兔多克隆抗体to PSMD1 -
宿主
Rabbit -
特异性
Detects proteasome Rpn2 from human cells. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human, African green monkey
预测可用于: Chicken
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免疫原
Synthetic peptide corresponding to Human PSMD1 aa 929-953.
Sequence:AAHGPKIEEEEQEPEPPEPFEYIDD
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract. |
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa). By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract. |
靶标
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功能
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
序列相似性
Belongs to the proteasome subunit S1 family.
Contains 10 PC repeats. - Information by UniProt
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数据库链接
- Entrez Gene: 424926 Chicken
- Entrez Gene: 5707 Human
- Entrez Gene: 70247 Mouse
- Entrez Gene: 83806 Rat
- SwissProt: Q5F418 Chicken
- SwissProt: Q99460 Human
- SwissProt: Q3TXS7 Mouse
- SwissProt: O88761 Rat
see all -
别名
- MGC133041 antibody
- 26S proteasome non ATPase regulatory subunit 1 antibody
- 26S proteasome non ATPase subunit 1 antibody
see all
图片
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Western blot - Anti-PSMD1 antibody (ab2941)All lanes : Anti-PSMD1 antibody (ab2941) at 1 µg/ml
Lane 1 : MCF7 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : SK-OV-3 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : COS-7 cell lysate
Lane 7 : Jurkat cell lysate
Lane 8 : A431 cell lysate
Lane 9 : Mouse testis tissue lysate
Lane 10 : Rat testis tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ HRP conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 105 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab2941 被引用在 8 文献中.
- Dekel E et al. 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun 12:1172 (2021). PubMed: 33608523
- Lu X et al. Structure-guided bifunctional molecules hit a DEUBAD-lacking hRpn13 species upregulated in multiple myeloma. Nat Commun 12:7318 (2021). PubMed: 34916494
- Osei-Amponsa V et al. Impact of Losing hRpn13 Pru or UCHL5 on Proteasome Clearance of Ubiquitinated Proteins and RA190 Cytotoxicity. Mol Cell Biol 40:N/A (2020). PubMed: 32631902
- Olshina MA et al. Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins. Antioxid Redox Signal 32:636-655 (2020). PubMed: 31903784
- Bhat K et al. Serum erythropoietin levels, breast cancer and breast cancer-initiating cells. Breast Cancer Res 21:17 (2019). PubMed: 30700319
- Chen X et al. Structure of hRpn10 Bound to UBQLN2 UBL Illustrates Basis for Complementarity between Shuttle Factors and Substrates at the Proteasome. J Mol Biol 431:939-955 (2019). PubMed: 30664872
- Lu X et al. Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Nat Commun 8:15540 (2017). PubMed: 28598414
- Moscovitz O et al. The Parkinson's-associated protein DJ-1 regulates the 20S proteasome. Nat Commun 6:6609 (2015). WB . PubMed: 25833141