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Anti-PSMC6抗体[p42-23] (ab22639)

Reviews (2) Submit a question References (5)
Western blot - Anti-PSMC6 antibody [p42-23] (ab22639)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC6 antibody [p42-23] (ab22639)
  • Flow Cytometry - Anti-PSMC6 antibody [p42-23] (ab22639)

Key features and details

  • Mouse monoclonal [p42-23] to PSMC6
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-PSMC6抗体[p42-23]
    参阅全部 PSMC6 一抗

  • 描述

    小鼠单克隆抗体[p42-23] to PSMC6

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, WB, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照

    • HeLa S3 cytosolic preparation and human placental proteasome preparation.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    Preservative: 0.065% Sodium azide
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Partially purified immunoglobulin preparation.

  • 克隆

    单克隆

  • 克隆编号

    p42-23

  • 同种型

    IgG2a

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab22639于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
WB (1)
Use at an assay dependent concentration. Detects a band of approximately 44 kDa.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
Flow Cyt
1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
WB
Use at an assay dependent concentration. Detects a band of approximately 44 kDa.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

  • 序列相似性

    Belongs to the AAA ATPase family.

  • 细胞定位

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 26S protease regulatory subunit 10B antibody
      • 26S protease regulatory subunit S10B antibody
      • 26S proteasome AAA-ATPase subunit RPT4 antibody
      • CADP44 antibody
      • P44 antibody
      • Proteasome 26S subunit ATPase 6 antibody
      • Proteasome subunit p42 antibody
      • PRS10_HUMAN antibody
      • PSMC6 antibody
      • Rpt4 antibody
      • SUG2 antibody
      see all

    图片

    • Western blot - Anti-PSMC6 antibody [p42-23] (ab22639)
      Western blot - Anti-PSMC6 antibody [p42-23] (ab22639)
      All lanes : Anti-PSMC6 antibody [p42-23] (ab22639) at 1/5000 dilution

      Lane 1 : Rpt4 in HeLa S3 cytosolic preparation
      Lane 2 : Purified protein
      Lane 3 : Proteasome in human placenta

      Developed using the ECL technique.

      Observed band size: 44 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC6 antibody [p42-23] (ab22639)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC6 antibody [p42-23] (ab22639)
      IHC image of ab22639 staining in human normal pancrease formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22639, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Flow Cytometry - Anti-PSMC6 antibody [p42-23] (ab22639)
      Flow Cytometry - Anti-PSMC6 antibody [p42-23] (ab22639)
      Overlay histogram showing HeLa cells stained with ab22639 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22639, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    文献 (5)

    发表研究结果有使用 ab22639？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab22639 被引用在 5 文献中.

    • Gizak A  et al. A novel remitting leukodystrophy associated with a variant in FBP2. Brain Commun 3:fcab036 (2021). PubMed: 33977262
    • Tao P  et al. Deubiquitination of proteasome subunits by OTULIN regulates type I IFN production. Sci Adv 7:eabi6794 (2021). PubMed: 34797715
    • Ahmed S  et al. Small-scale isolation of synaptic vesicles from mammalian brain. Nat Protoc 8:998-1009 (2013). PubMed: 23619891
    • Rousseau E  et al. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem 284:1917-29 (2009). WB, ICC/IF ; Human . PubMed: 18986984
    • Hendil KB  et al. Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes. Biochem J 332 ( Pt 3):749-54 (1998). PubMed: 9620878

    客户评价及客户问答

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    Western blot abreview for Anti-PSMC6 antibody [p42-23]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (liver)
    Loading amount
    30 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (4-20% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    提交于 Dec 13 2010

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