重组Anti-PSMC5抗体[EPR13566(B)] - C-terminal (ab180495)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13566(B)] to PSMC5 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PSMC5抗体[EPR13566(B)] - C-terminal
参阅全部 PSMC5 一抗 -
描述
兔单克隆抗体[EPR13566(B)] to PSMC5 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human PSMC5 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P62195 -
阳性对照
- HepG2, HeLa and 293T lysates; Human pancreas tissue; 293T and HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13566(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180495于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
|
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ICC/IF |
1/500.
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
说明 |
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Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). |
ICC/IF
1/500. |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. -
序列相似性
Belongs to the AAA ATPase family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5705 Human
- Entrez Gene: 19184 Mouse
- Entrez Gene: 81827 Rat
- Omim: 601681 Human
- SwissProt: P62195 Human
- SwissProt: P62196 Mouse
- SwissProt: P62198 Rat
- Unigene: 79387 Human
see all -
别名
- 26S protease regulatory subunit 8 antibody
- 26S proteasome AAA-ATPase subunit RPT6 antibody
- Cim3 antibody
see all
图片
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All lanes : Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 46 kDa -
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PSMC5 with ab180495 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed)labeling PSMC5 with ab180495 at 1/30 dilution (green) compared to a negative control Rabbit monoclonal IgG (red) follwed byGoat anti rabbit IgG (FITC) secondary at 1/75 dilution.
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Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling PSMC5 with ab180495 at 1/250 dilution (red) followed by with Goat anti rabbit IgG (Dylight 555) . DAPI staining (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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