Anti-PSMA3抗体(ab180784)
Key features and details
- Rabbit polyclonal to PSMA3
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-PSMA3抗体
参阅全部 PSMA3 一抗 -
描述
兔多克隆抗体to PSMA3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human PSMA3 aa 1-255.
Sequence:MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGV EKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFR SNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLY MIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYI VHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDES DDDNM
Database link: P25788 -
阳性对照
- JAR, A549 and PC12 cell extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180784于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 28 kDa.
|
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| ICC/IF |
1/50 - 1/200.
|
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| IP |
1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 28 kDa. |
|
ICC/IF
1/50 - 1/200. |
|
IP
1/1000. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2. -
序列相似性
Belongs to the peptidase T1A family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5684 Human
- Entrez Gene: 19167 Mouse
- Entrez Gene: 29670 Rat
- Entrez Gene: 408248 Rat
- Omim: 176843 Human
- SwissProt: P25788 Human
- SwissProt: O70435 Mouse
- SwissProt: P18422 Rat
see all -
别名
- HC8 antibody
- Macropain subunit C8 antibody
- MGC12306 antibody
see all
图片
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Western blot - Anti-PSMA3 antibody (ab180784)All lanes : Anti-PSMA3 antibody (ab180784) at 1/1000 dilution
Lane 1 : MCF7 cell lysate with 3% nonfat dry milk in TBST
Lane 2 : DU145 cell lysate with 3% nonfat dry milk in TBST
Lane 3 : HL-60 cell lysate with 3% nonfat dry milk in TBST
Lane 4 : PC-3 cell lysate with 3% nonfat dry milk in TBST
Lane 5 : Mouse liver lysate with 3% nonfat dry milk in TBST
Lane 6 : Mouse spleen lysate with 3% nonfat dry milk in TBST
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa -
Immunoprecipitation - Anti-PSMA3 antibody (ab180784)Immunoprecipitation analysis of 200 μg extracts of HL-60 cells, using 3 μg of ab180784. Western blot was performed from the immunoprecipitate using ab180784 at a dilition of 1:1000.
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Western blot - Anti-PSMA3 antibody (ab180784)All lanes : Anti-PSMA3 antibody (ab180784) at 1/500 dilution
Lane 1 : JAR cell extracts
Lane 2 : A549 cell extracts
Lane 3 : PC12 cell extracts
Predicted band size: 28 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PSMA3 antibody (ab180784)Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180784. Blue DAPI for nuclear staining.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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