重组Anti-PSMA抗体[SP29] (ab64082)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [SP29] (ab64082)](https://www.abcam.cn/ps/products/64/ab64082/Images/IHC PSMA.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [SP29] (ab64082)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP29] to PSMA
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PSMA抗体[SP29]
参阅全部 PSMA 一抗 -
描述
兔单克隆抗体[SP29] to PSMA -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
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阳性对照
- Human prostate carcinoma
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常规说明
ab64082 was switched from a hybridoma to recombinant production method on 17th September 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SP29 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-PSMA antibody [SP29] - BSA and Azide free (ab245747)
- Alexa Fluor® 488 Anti-PSMA antibody [SP29] (ab320545)
- Alexa Fluor® 647 Anti-PSMA antibody [SP29] (ab320546)
- Alexa Fluor® 555 Anti-PSMA antibody [SP29] (ab320547)
- Alexa Fluor® 594 Anti-PSMA antibody [SP29] (ab320548)
- PE Anti-PSMA antibody [SP29] (ab320549)
- APC Anti-PSMA antibody [SP29] (ab320550)
- Alexa Fluor® 750 Anti-PSMA antibody [SP29] (ab321686)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64082于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
| 说明 |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 1/100 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
靶标
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功能
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. -
组织特异性
Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer. -
序列相似性
Belongs to the peptidase M28 family. M28B subfamily. -
结构域
The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal. -
翻译后修饰
The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked. -
细胞定位
Cytoplasm and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2346 Human
- Omim: 600934 Human
- SwissProt: Q04609 Human
- Unigene: 654487 Human
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别名
- Cell growth inhibiting protein 27 antibody
- Cell growth-inhibiting gene 27 protein antibody
- FGCP antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [SP29] (ab64082)
Immunohistochemical analysis of PSMA expression in paraffin embedded formalin fixed human prostate carcinoma tissue using ab64082 at a 1/100 dilution.
This image was generated from the hybridoma version.
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![Anti-PSMA antibody [SP29] (ab64082)](https://www.abcam.cn/ps/products/64/ab64082/Images/ab64082-2-benefits-of-recombinant-antibodies.png)
Anti-PSMA antibody [SP29] (ab64082)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab64082 被引用在 2 文献中.
- Russo GI et al. Genomic Landscape Alterations in Primary Tumor and Matched Lymph Node Metastasis in Hormone-Naïve Prostate Cancer Patients. Cancers (Basel) 14:N/A (2022). PubMed: 36077746
- Bravaccini S et al. PSMA expression: a potential ally for the pathologist in prostate cancer diagnosis. Sci Rep 8:4254 (2018). IHC-P . PubMed: 29523813