重组Anti-PSMA抗体[EPR6253] (ab133579)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6253] to PSMA
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PSMA抗体[EPR6253]
参阅全部 PSMA 一抗 -
描述
兔单克隆抗体[EPR6253] to PSMA -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human PSMA aa 1-150 (N terminal). The exact sequence is proprietary.
Database link: Q04609 -
阳性对照
- Human prostate carcinoma tissue; LNCaP cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6253 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133579于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/50000 - 1/200000. Predicted molecular weight: 84 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/50000 - 1/200000. Predicted molecular weight: 84 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. -
组织特异性
Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer. -
序列相似性
Belongs to the peptidase M28 family. M28B subfamily. -
结构域
The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal. -
翻译后修饰
The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked. -
细胞定位
Cytoplasm and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2346 Human
- Omim: 600934 Human
- SwissProt: Q04609 Human
- Unigene: 654487 Human
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别名
- Cell growth inhibiting protein 27 antibody
- Cell growth-inhibiting gene 27 protein antibody
- FGCP antibody
see all
图片
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Anti-PSMA antibody [EPR6253] (ab133579) at 1/100000 dilution (Purified) + LNCaP lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L)
Predicted band size: 84 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?5% NFDM/TBST dilution buffer
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)
Immunohistochemical staining of paraffin embedded human prostate cancer with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)
Unpurified ab133579 showing positive staining in Prostatic carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Primary: ab133579, 1/50 dilution
Dilution buffer: NFDM/TBST, 5%
Sample: LNCaP
Secondary: HRP goat anti-rabbit (H+L), 1/1000 dilution
Anti-PSMA antibody [EPR6253] (ab133579) at 1/50 dilution + LNCaP with NFDM/TBST at 5 %
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Anti-PSMA antibody [EPR6253] (ab133579) at 1/50000 dilution (Unpurified) + LNCaP cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)
Unpurified ab133579 showing positive staining in Normal prostate tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)
Unpurified ab133579 showing negative staining in Thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)
Unpurified ab133579 showing negative staining in Normal colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)
Unpurified ab133579 showing negative staining in Breast carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)
Unpurified ab133579 showing negative staining in Normal spleen tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab133579 被引用在 21 文献中.
- Wang X et al. High GLUT1 membrane expression and low PSMA membrane expression in Ductal Adenocarcinoma and Intraductal Carcinoma of the prostate. Prostate Cancer Prostatic Dis N/A:N/A (2023). PubMed: 38007533
- Zou S et al. Time point-independent tumor positivity of 68Ga-PSMA-PET/CT pre- and post-biopsy in high-risk prostate cancer. Ann Nucl Med 36:523-532 (2022). PubMed: 35362891
- Piron S et al. Preclinical comparative study of [18F]AlF-PSMA-11 and [18F]PSMA-1007 in varying PSMA expressing tumors. Sci Rep 12:15744 (2022). PubMed: 36130980
- Wu L et al. The establishment of polypeptide PSMA-targeted chimeric antigen receptor-engineered natural killer cells for castration-resistant prostate cancer and the induction of ferroptosis-related cell death. Cancer Commun (Lond) 42:768-783 (2022). PubMed: 35706368
- Tu W et al. Secreted phosphoprotein 1 promotes angiogenesis of glioblastoma through upregulating PSMA expression via transcription factor HIF1α. Acta Biochim Biophys Sin (Shanghai) 55:417-425 (2022). PubMed: 36305723