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AB133579

重组Anti-PSMA抗体[EPR6253]

Anti-PSMA antibody [EPR6253]

3

(2 Reviews)

|

(29 Publications)

Anti-PSMA antibody [EPR6253] (ab133579) is a rabbit monoclonal antibody detecting PSMA in Western Blot, IP, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

查看别名

FOLH, NAALAD1, PSM, PSMA, GIG27, FOLH1, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase, FGCP, GCPII, mGCP, NAALADase I

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)

Unpurified ab133579 showing positive staining in Prostatic carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)

Unpurified ab133579 showing positive staining in Normal prostate tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)

Immunohistochemical staining of paraffin embedded human prostate cancer with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)

Unpurified ab133579 showing negative staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-PSMA antibody [EPR6253] (AB133579)
  • IP

Lab

Immunoprecipitation - Anti-PSMA antibody [EPR6253] (AB133579)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Secondary : HRP goat anti-rabbit (H+L), 1/1000 dilution

All lanes:

Immunoprecipitation - Anti-PSMA antibody [EPR6253] (ab133579) at 1/50 dilution

All lanes:

LNCaP

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 84 kDa

Observed band size: 110 kDa

false

Western blot - Anti-PSMA antibody [EPR6253] (AB133579)
  • WB

Supplier Data

Western blot - Anti-PSMA antibody [EPR6253] (AB133579)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PSMA antibody [EPR6253] (ab133579) at 1/50000 dilution

All lanes:

LnCap (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 110 kDa

false

Exposure time: 40s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)

Unpurified ab133579 showing negative staining in Thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)

Unpurified ab133579 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)

Unpurified ab133579 showing negative staining in Normal spleen tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

不同偶联物与剂型 (4)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6253

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IP, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

What is this antibody validated in?
Anti-PSMA antibody [EPR6253] (ab133579) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of PSMA?
Anti-PSMA [EPR6253] (ab133579) specifically detects a band for PSMA (UniProt: Q04609) at a molecular weight of 84kDa.

Trusted by the scientific community
Anti-PSMA [EPR6253] (ab133579) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR6253] also available for your convenience: ab133579, Biotin - ab201253, Carrier free - ab226141, Alexa Fluor® 488 - ab309608, Alexa Fluor® 647 - ab313352

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
See full target information FOLH1
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文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:9558-9579 PubMed41041045

2025

Luminal Stem-like Cells in High-Risk/Locally Advanced Prostate Cancer Promote Resistance to Hormonal Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Guyu Tang,Jing Liu,Xiaomei Gao,Kai Yuan,Minfeng Chen,Long Wang,Lin Qi,Yi Cai

Tomography (Ann Arbor, Mich.) 11: PubMed40137569

2025

Preclinical Evaluation of a Novel PSMA-Targeted Agent Ga-NOTA-GC-PSMA for Prostate Cancer Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjin Li,Yihui Luo,Yuqi Hua,Qiaoling Shen,Liping Chen,Yu Xu,Haitian Fu,Chunjing Yu

Journal for immunotherapy of cancer 13: PubMed40121032

2025

Inhibition of stromal MAOA leading activation of WNT5A enhance prostate cancer immunotherapy by involving the transition of cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zhite Zhao,Yaohua Hu,Hui Li,Tong Lu,Xinglin He,Yifan Ma,Minli Huang,Mengyao Li,Lijun Yang,Changhong Shi

Journal of nanobiotechnology 22:803 PubMed39734237

2024

Targeting AURKA with multifunctional nanoparticles in CRPC therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Deng,Binghu Ke,Qixing Tian,Yukui Gao,Qiliang Zhai,Wenqiang Zhang

BMC cancer 24:1328 PubMed39472809

2024

Potential of PSMA for breast cancer in nuclear medicine: digital quantitative immunohistochemical analysis and implications for a theranostic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Zoé Neviere,Cécile Blanc-Fournier,Anne-Valérie Guizard,Nicolas Elie,Florence Giffard,Justine Lequesne,George Emile,Laurent Poulain,Charline Lasnon

American journal of nuclear medicine and molecular imaging 14:13-21 PubMed38500747

2024

Molecular imaging reveals the heterogeneous progression of tumor cells and tumor stroma: a practice of FDG PET and FAPI PET in diagnosing PSMA-negative bone metastases of progressive prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhi Zhu,Peng Chen,Zhongqiu Guo,Fangdu Li,Xiu Luo,Xia Du,Liying Zhang,Changjing Zuo,Xiao Li

Prostate cancer and prostatic diseases : PubMed38007533

2023

High GLUT1 membrane expression and low PSMA membrane expression in Ductal Adenocarcinoma and Intraductal Carcinoma of the prostate.

Applications

Unspecified application

Species

Unspecified reactive species

Xingming Wang,Li Zhou,Lin Qi,Ye Zhang,Hongling Yin,Yu Gan,Xiaomei Gao,Yi Cai

American journal of nuclear medicine and molecular imaging 13:107-117 PubMed37457328

2023

Co-injection of anti-HER2 antibody Trastuzumab does not increase efficacy of [Lu]Lu-PSMA-617 therapy in an animal model of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ayman Abouzayed,Wahed Zedan,Mohamed Altai,Joanna Strand,Anders Örbom

Translational oncology 28:101616 PubMed36621073

2023

The clinical application of Ga-PSMA PET/CT and regulating mechanism of PSMA expression in patients with brain metastases of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchen Pei,Chang Liu,Mingtao Feng,Liangdong Li,Changshuai Zhou,Lei Chen,Xin Hu,Shaoli Song,Yiqun Cao,Yang Gao

Acta biochimica et biophysica Sinica 55:417-425 PubMed36305723

2022

Secreted phosphoprotein 1 promotes angiogenesis of glioblastoma through upregulating PSMA expression via transcription factor HIF1α.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Tu,Hui Zheng,Liangdong Li,Changshuai Zhou,Mingtao Feng,Lei Chen,Deheng Li,Xin Chen,Bin Hao,Huaping Sun,Yiqun Cao,Yang Gao
View all publications
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