Anti-PSF1抗体(ab183524)
Key features and details
- Rabbit polyclonal to PSF1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PSF1抗体
参阅全部 PSF1 一抗 -
描述
兔多克隆抗体to PSF1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chicken, Turkey, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan -
免疫原
Synthetic peptide within Human PSF1 aa 146-196. The exact sequence is proprietary. NP_066545.3.
Database link: Q14691 -
阳性对照
- HeLa, 293T and Jurkat cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab183524 was affinity purified using an epitope specific to PSF1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183524于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 23 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 23 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function. -
序列相似性
Belongs to the GINS1/PSF1 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 425297 Chicken
- Entrez Gene: 458147 Chimpanzee
- Entrez Gene: 100413181 Common marmoset
- Entrez Gene: 101127635 Gorilla
- Entrez Gene: 9837 Human
- Entrez Gene: 102131465 Macaque monkey
- Entrez Gene: 100450573 Orangutan
- Omim: 610608 Human
see all -
别名
- DNA replication complex GINS protein PSF1 antibody
- GINS complex subunit 1 (Psf1 homolog) antibody
- GINS complex subunit 1 antibody
see all
图片
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Detection of PSF1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab183524 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab183524 was used at 1 µg/ml. Lane 2 represents control IgG IP.
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All lanes : Anti-PSF1 antibody (ab183524) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 23 kDa
Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab183524 被引用在 2 文献中.
- McQuaid ME et al. Hypomorphic GINS3 variants alter DNA replication and cause Meier-Gorlin syndrome. JCI Insight 7:N/A (2022). PubMed: 35603789
- Yang H et al. GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A. iScience 25:104952 (2022). PubMed: 36065190