重组Anti-PSD93抗体[EPR26468-112]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (AB289972)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling PSD93 with ab289972 at 1/500 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab289972 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS instead of primary antibody, followed by LeicaDS9800 (Bond_^™, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (AB289972)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling PSD93 with ab289972 at 1/500 (1.222 followed by LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control : No staining on human cardiac muscle. The section was incubated with ab289972 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.Counterstained with Hematoxylin..

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IP

Lab

Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (AB289972)

PSD93 was immunoprecipitated from SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab289972 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289972 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab289972 IP in SH-SY5Y whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289972 in SH-SY5Y whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (ab289972)

Predicted band size: 97 kDa

Observed band size: 25-110 kDa

false

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (AB289972)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling PSD93 with ab289972 at 1/500 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab289972 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.Counterstained with Hematoxylin.

Secondary antibody only control : PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD93 antibody [EPR26468-112] (AB289972)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling PSD93 with ab289972 at 1/500 followed by LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab289972 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Lab

Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (AB289972)

PSD93 was immunoprecipitated from Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab289972 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289972 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

Lane 2 : ab289972 IP in Neuro-2a whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289972 in Neuro-2a whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (ab289972)

Predicted band size: 97 kDa

Observed band size: 25-110 kDa

false

• WB

Lab

Western blot - Anti-PSD93 antibody [EPR26468-112] (AB289972)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

DLG2 has many splicing forms. The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 14724236).

Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-PSD93 antibody [EPR26468-112] (ab289972) at 1/1000 dilution

All lanes:

Human cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 97 kDa

Observed band size: 25-110 kDa

false

• WB

Lab

Western blot - Anti-PSD93 antibody [EPR26468-112] (AB289972)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

DLG2 has many splicing forms. The expression profile/molecular weight observed is consistent with that described in the literature (PMID : 14724236).

Negative control : spleen (PMID : 7590743)

Exposure time : 15 seconds

All lanes:

Western blot - Anti-PSD93 antibody [EPR26468-112] (ab289972) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

Observed band size: 25-110 kDa

false

• WB

Lab

Western blot - Anti-PSD93 antibody [EPR26468-112] (AB289972)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody does not cross-react with human DLG1.

Both recombinant proteins were made in-house and expressed from E.coli expression systems.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-PSD93 antibody [EPR26468-112] (ab289972) at 1/1000 dilution

Lane 1:

His-tagged Human DLG2 recombinant protein (20ng)

Lane 2:

His-tagged Human DLG1 recombinant protein (20ng)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

Observed band size: 25-110 kDa

false