Anti-PRR11抗体[OTI1A4] (ab131652)
Key features and details
- Mouse monoclonal [OTI1A4] to PRR11
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-PRR11抗体[OTI1A4]
参阅全部 PRR11 一抗 -
描述
小鼠单克隆抗体[OTI1A4] to PRR11 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant fragment corresponding to Human PRR11 aa 125-360. (NP_060774) produced in E.coli.
Sequence:MPKFKQRRRKLKAKAERLFKKKEASHFQSKLITPPPPPPSPERVGISSID ISQSRSWLTSSWNFNFPNIRDAIKLWTNRVWSIYSWCQNCITQSLEVLKD TIFPSRICHRELYSVKQQFCILESKLCKLQEALKTISESSSCPSCGQTCH MSGKLTNVPACVLITPGDSKAVLPPTLPQPASHFPPPPPPPPLPPPPPPL APVLLRKPSLAKALQAGPLKKDGPMQITVKDLLTVKLKKTQSLDEKRKLI PSPKARNPLVTVSDLQHVTLKPNSKVLSTRVTNVLITPGKSQMDLRKLLR KVDVERSPGGTPLTNKENMETGTGLTPVMTQALRRKFQLAHPRSPTPTLP LSTSSFDEQN
Database link: Q96HE9 -
阳性对照
- WB: HEK-293T cells transfected with pCMV6-ENTRY PRR11 cDNA; HepG2, HeLa, SVT2, A549, COS-7, jurkat, MDCK, PC-12 and MCF7 whole cell lysate.
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常规说明
The clone number has been updated from 1A4 to OTI1A4, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI1A4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131652于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000. Predicted molecular weight: 40 kDa.
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说明 |
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WB
1/2000. Predicted molecular weight: 40 kDa. |
靶标
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翻译后修饰
Ubiquitinated (Probable). Rapidly degraded by the proteasome; degradation may involve FBXW7-specific phosphorylated phosphodegron motifs. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 55771 Human
- Omim: 615920 Human
- SwissProt: Q96HE9 Human
- Unigene: 631750 Human
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别名
- FLJ11029 antibody
- Homo sapiens proline rich 11 antibody
- Proline rich protein 11 antibody
see all
图片
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All lanes : Anti-PRR11 antibody [OTI1A4] (ab131652) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SVT2 whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK whole cell lysate
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 40 kDa -
All lanes : Anti-PRR11 antibody [OTI1A4] (ab131652)
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PRR11 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 40 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201889)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab131652 尚未被引用在任何文献中。