Anti-PRPF8/Prp8抗体[2834C1a] (ab51366)
![Western blot - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)](https://www.abcam.cn/ps/products/51/ab51366/Images/ab51366wb.jpg "Western blot - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)")
Key features and details
- Mouse monoclonal [2834C1a] to PRPF8/Prp8
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-PRPF8/Prp8抗体[2834C1a]
参阅全部 PRPF8/Prp8 一抗 -
描述
小鼠单克隆抗体[2834C1a] to PRPF8/Prp8 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human PRPF8/Prp8.
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阳性对照
- NIH3T3 whole cell lysate, HeLa whole cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cervix.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Filtered through a 0.22 µm membrane. -
克隆
单克隆 -
克隆编号
2834C1a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51366于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/100. Predicted molecular weight: 273 kDa.
|
|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
1/100. Predicted molecular weight: 273 kDa. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes. -
组织特异性
Widely expressed. -
疾病相关
Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant. -
序列相似性
Contains 1 MPN (JAB/Mov34) domain. -
结构域
The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus speckle. - Information by UniProt
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数据库链接
- Entrez Gene: 10594 Human
- Entrez Gene: 192159 Mouse
- Omim: 607300 Human
- SwissProt: Q6P2Q9 Human
- SwissProt: Q99PV0 Mouse
- Unigene: 181368 Human
- Unigene: 3757 Mouse
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别名
- 220 kDa U5 snRNP specific protein antibody
- 220 kDa U5 snRNP-specific protein antibody
- Apoptosis regulated protein 1 antibody
see all
图片
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Western blot - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 273 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)](https://www.abcam.cn/ps/products/51/ab51366/Images/ab51366-195979-ab51366gr198315edited.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)IHC image of PRPF8/Prp8 staining in Human Normal Cervix formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51366, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)](https://www.abcam.cn/ps/products/51/ab51366/Images/ab51366wb2.jpg)
Western blot - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 273 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted? -
![Flow Cytometry - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)](https://www.abcam.cn/ps/products/51/ab51366/Images/PRPF8-Primary-antibodies-ab51366-1.jpg)
Flow Cytometry - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)Overlay histogram showing HeLa cells stained with ab51366 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51366, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab51366 被引用在 2 文献中.
- Pederiva C et al. Splicing controls the ubiquitin response during DNA double-strand break repair. Cell Death Differ 23:1648-57 (2016). PubMed: 27315300
- Maxwell SS et al. Chromatin context and ncRNA highlight targets of MeCP2 in brain. RNA Biol 10:1741-57 (2013). PubMed: 24270455
