JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190347

重组Anti-PRPF8/Prp8抗体[EPR15228]

Anti-PRPF8/Prp8 antibody [EPR15228]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal PRPF8/Prp8 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

查看别名

PRPC8, PRPF8, Pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling PRPF8/Prp8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PRPF8/Prp8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)
  • WB

Supplier Data

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)

All lanes:

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 274 kDa

Observed band size: 274 kDa

true

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)
  • WB

Supplier Data

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)

All lanes:

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/20000 dilution

All lanes:

293T cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 274 kDa

Observed band size: 274 kDa

true

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)
  • WB

Supplier Data

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (AB190347)

All lanes:

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/1000 dilution

Lane 1:

mouse RAW 264.7 cell lysate at 10 µg

Lane 2:

rat PC12 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 274 kDa

Observed band size: 274 kDa

true

不同偶联物与剂型 (1)

  • Carrier free

    Anti-PRPF8/Prp8 antibody [EPR15228] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15228

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

替代产品

Primary Antibodies

AB245504

Anti-PRPF8/Prp8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody (AB245504)

  • 0
  • 0
Primary Antibodies

AB185547

Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - C-terminal (AB185547)

  • 4
  • 1
Primary Antibodies

AB239003

Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (AB239003)

  • 0
  • 0
Primary Antibodies

AB51366

Anti-PRPF8/Prp8 antibody [2834C1a]

BOND RX™ Validated

Flow Cytometry - Anti-PRPF8/Prp8 antibody [2834C1a] (AB51366)

  • 0
  • 0
Primary Antibodies

AB79237

Anti-PRPF8/Prp8 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody (AB79237)

  • 5
  • 5

搭配使用产品

Primary Antibodies

AB176715

Anti-SNRNP200 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRNP200 antibody (AB176715)

  • 0
  • 0
Primary Antibodies

AB188327

Anti-EFTUD2 antibody [EPR16055(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFTUD2 antibody [EPR16055(B)] (AB188327)

  • 0
  • 0
Primary Antibodies

AB240636

Anti-Ctip2 antibody [EPR23120-25]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] (AB240636)

  • 5
  • 1
Primary Antibodies

AB204916

Anti-SC35 antibody [EPR12238]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SC35 antibody [EPR12238] (AB204916)

  • 4
  • 2
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome (PubMed : 10411133, PubMed : 11971955, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 29301961, PubMed : 29360106, PubMed : 29361316, PubMed : 30315277, PubMed : 30705154, PubMed : 30728453). Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
See full target information PRPF8
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 31:747-751 PubMed38467876

2024

Structural basis of human U5 snRNP late biogenesis and recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Riabov Bassat,Supapat Visanpattanasin,Matthias K Vorländer,Laura Fin,Alexander W Phillips,Clemens Plaschka

RNA biology 15:115-129 PubMed29023197

2017

Mutational landscape of RNA-binding proteins in human cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Yaseswini Neelamraju,Abel Gonzalez-Perez,Poornima Bhat-Nakshatri,Harikrishna Nakshatri,Sarath Chandra Janga
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com