AB198998

重组Anti-PRPF4抗体[EPR17207] - C-terminal

Anti-PRPF4 antibody [EPR17207] - C-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PRPF4 antibody. C-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

查看别名

PRP4, PRPF4, U4/U6 small nuclear ribonucleoprotein Prp4, PRP4 homolog, U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4, hPrp4

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Human kidney tissue was observed (Subcellular location - Nucleus speckle [UniProt]).
Negative control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Human transitional cell carcinoma of bladder tissue was observed (Subcellular location - Nucleus speckle [UniProt]).

Negative control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Intracellular Flow Cytometry analysis of HeLa cells labelling PRPF4 (red) with purified ab198998 at dilution of 1/70. The secondary antibody used was Alexa Fluorr^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Rat cerebral cortex tissue was observed (Subcellular location - Nucleus speckle [UniProt]).
Negative control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Mouse cardiac muscle tissuewas observed (Subcellular location - Nucleus speckle [UniProt]).
Negative control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Blocking and diluting buffer was 5% NFDM/TBST

All lanes:

Western blot - Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/20000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma) whole cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

Lane 3:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Lane 4:

HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Blocking and diluting buffer was 5% NFDM/TBST

All lanes:

Western blot - Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor) whole cell lysate at 10 µg

Lane 2:

Raw264.7 (Mouse macrophages transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

true

Exposure time: 15s

Supplier Data

Western blot - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Blocking and diluting buffer was 5% NFDM/TBST

All lanes:

Western blot - Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Lane 3:

Mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

true

Exposure time: 3min

不同偶联物与剂型 (1)

Carrier free

Anti-PRPF4 antibody [EPR17207] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17207

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/70", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).

See full target information PRPF4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell stress & chaperones 30:100084 PubMed40412548

2025

Heat shock induces alternative polyadenylation through dynamic DNA methylation and chromatin looping.

Applications

Unspecified application

Species

Unspecified reactive species

Emily E Fink,Yi Zhang,Briana Santo,Anwita Siddavatam,Rosie Ou,Vishal Nanavaty,Byron H Lee,Angela H Ting

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-PRPF4抗体[EPR17207] - C-terminal

Anti-PRPF4 antibody [EPR17207] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Flow Cytometry (Intracellular) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Western blot - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Western blot - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)

Western blot - Anti-PRPF4 antibody [EPR17207] - C-terminal (AB198998)