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重组RabMAb

重组Anti-PRP19抗体[EPR7446] (ab126776)

Submit a review Submit a question References (3)
Western blot - Anti-PRP19 antibody [EPR7446] (ab126776)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776)
  • OI-RD Scanning - Anti-PRP19 antibody [EPR7446] (ab126776)
  • Anti-PRP19 antibody [EPR7446] (ab126776)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7446] to PRP19
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-PRP19抗体[EPR7446]
    参阅全部 PRP19 一抗

  • 描述

    兔单克隆抗体[EPR7446] to PRP19

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human PRP19 aa 450-550. The exact sequence is proprietary.

  • 阳性对照

    • Human breast carcinoma and colon carcinoma tissues; HepG2, A431, MCF7, PC12, NIH 3T3, HeLa, K562, and U937 cell lysates.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126776于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.

  • 组织特异性

    Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.

  • 序列相似性

    Belongs to the WD repeat PRP19 family.
    Contains 1 U-box domain.
    Contains 7 WD repeats.

  • 细胞定位

    Nucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • hPso4 antibody
    • NMP200 antibody
    • Nuclear matrix protein 200 antibody
    • Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody
    • pre mRNA processing factor 19 antibody
    • Pre-mRNA-processing factor 19 antibody
    • PRP19 antibody
    • PRP19/PSO4 homolog antibody
    • PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody
    • PRP19_HUMAN antibody
    • PRPF19 antibody
    • PSO4 antibody
    • psoralen 4 antibody
    • Senescence evasion factor antibody
    • SNEV antibody
    • UBOX4 antibody
    see all

图片

  • Western blot - Anti-PRP19 antibody [EPR7446] (ab126776)
    Western blot - Anti-PRP19 antibody [EPR7446] (ab126776)
    All lanes : Anti-PRP19 antibody [EPR7446] (ab126776) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : PC-12 cell lysate
    Lane 5 : NIH/3T3 cell lysate
    Lane 6 : HeLa cell lysate
    Lane 7 : K562 cell lysate
    Lane 8 : U937 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776)

    ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] (ab126776)

    ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-PRP19 antibody [EPR7446] (ab126776)
    OI-RD Scanning - Anti-PRP19 antibody [EPR7446] (ab126776)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PRP19 antibody [EPR7446] (ab126776)
    Anti-PRP19 antibody [EPR7446] (ab126776)

文献 (3)

发表研究结果有使用 ab126776？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab126776 被引用在 3 文献中.

  • Wang Y  et al. Comprehensive analysis of PRPF19 immune infiltrates, DNA methylation, senescence-associated secretory phenotype and ceRNA network in bladder cancer. Front Immunol 14:1289198 (2023). PubMed: 38022515
  • Cai Y  et al. Prp19 Is an Independent Prognostic Marker and Promotes Neuroblastoma Metastasis by Regulating the Hippo-YAP Signaling Pathway. Front Oncol 10:575366 (2020). PubMed: 33224878
  • Guo J  et al. Unraveling molecular effects of ADAR1 overexpression in HEK293T cells by label-free quantitative proteomics. Cell Cycle 15:1591-601 (2016). PubMed: 27104882

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