Anti-PROX1抗体(ab38692)
Key features and details
- Rabbit polyclonal to PROX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PROX1抗体
参阅全部 PROX1 一抗 -
描述
兔多克隆抗体to PROX1 -
宿主
Rabbit -
特异性
We have had mixed results for use of this antibody in IHC-P. The AbReview below from an external researcher reports good staining with this antibody with human heart tissue, but we have had mixed results with this antibody in internal QC testing in IHC-P. We welcome any feedback from customers who have used this antibody in IHC-P.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human PROX1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab38691) -
阳性对照
- WB: HepG2, SHSY5Y whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab38692于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).
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说明 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa). |
靶标
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功能
Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3. -
组织特异性
Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas. -
序列相似性
Belongs to the Prospero homeobox family.
Contains 1 Prospero-type homeobox DNA-binding domain. -
结构域
The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity. -
细胞定位
Nucleus. RORG promotes its nuclear localization. - Information by UniProt
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数据库链接
- Entrez Gene: 5629 Human
- Entrez Gene: 19130 Mouse
- Entrez Gene: 305066 Rat
- Omim: 601546 Human
- SwissProt: Q92786 Human
- SwissProt: P48437 Mouse
- Unigene: 741808 Human
- Unigene: 744931 Human
see all -
形式
Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter. -
别名
- Homeobox prospero like protein antibody
- Homeobox prospero like protein PROX1 antibody
- Homeobox prospero-like protein PROX1 antibody
see all
图片
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All lanes : Anti-PROX1 antibody (ab38692) at 1 µg/ml
Lane 1 : HepG2 whole cell lysate
Lane 2 : SHSY5Y whole cell lysate
Lane 3 : Hek293 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 83 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesGel type: MOPS
Blocking buffer: 3% milk
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab38692 被引用在 15 文献中.
- Jo M et al. Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping. Microcirculation 30:e12788 (2023). PubMed: 36169611
- Genescu I et al. Dynamic interplay between thalamic activity and Cajal-Retzius cells regulates the wiring of cortical layer 1. Cell Rep 39:110667 (2022). PubMed: 35417707
- Koike H et al. Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells. Nat Protoc 16:919-936 (2021). PubMed: 33432231
- Taha E et al. eEF2/eEF2K Pathway in the Mature Dentate Gyrus Determines Neurogenesis Level and Cognition. Curr Biol 30:3507-3521.e7 (2020). PubMed: 32707059
- Shibata-Germanos S et al. Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges. Acta Neuropathol 139:383-401 (2020). PubMed: 31696318