Anti-Protein CASP抗体[2A10] - BSA and Azide free (ab54583)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)](https://www.abcam.cn/ps/products/54/ab54583/Images/ab54583-212606-anti-protein-casp-antibody-2a10-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)")
Key features and details
- Mouse monoclonal [2A10] to Protein CASP - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-Protein CASP抗体[2A10] - BSA and Azide free
参阅全部 Protein CASP 一抗 -
描述
小鼠单克隆抗体[2A10] to蛋白CASP - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cytmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment (GST-tag) corresponding to Human Protein CASP aa 500-650. NP_001904.2
Database link: Q13948 -
阳性对照
- ICC/IF: HeLa cells. IHC-P: Human spleen tissue; human malignant lymphoma, diffuse large B tissue. Flow Cytometry: MCF7 cells. WB: Protein CASP transfected HEK-293T cell lysate. Recombinant Protein CASP protein.
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常规说明
This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
2A10 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54583于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 77.5 kDa.
|
| ICC/IF | (3) |
Use at an assay dependent concentration.
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| Sandwich ELISA |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 77.5 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
|
Sandwich ELISA
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
May be involved in intra-Golgi retrograde transport. -
序列相似性
Belongs to the CASP family. -
细胞定位
Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1523 Human
- SwissProt: Q13948 Human
- Unigene: 191482 Human
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别名
- CASP_HUMAN antibody
- CUX1 antibody
- Protein CASP antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)
Formalin-fixed, paraffin-embedded human spleen tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)](https://www.abcam.cn/ps/products/54/ab54583/Images/ab54583-67509-anti-protein-casp-antibody-2a10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Protein CASP (green) using ab54583 at 10 µg/ml in ICC/IF.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)](https://www.abcam.cn/ps/products/54/ab54583/Images/ab54583-67508-anti-protein-casp-antibody-2a10-flow-cytometry.jpg)
Flow Cytometry - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)Overlay histogram showing MCF7 (human breast adenocarcinoma cell line) cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3 M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1 µg/1 x 106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)](https://www.abcam.cn/ps/products/54/ab54583/Images/ab54583-212600-anti-protein-casp-antibody-2a10-western-blot.jpg)
Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)All lanes : Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/ml
Lane 1 : Protein CASP transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 77.5 kDaThis image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)](https://www.abcam.cn/ps/products/54/ab54583/Images/ab54583-67511-anti-protein-casp-antibody-2a10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)Formalin-fixed, paraffin-embedded human malignant lymphoma, diffuse large B tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)](https://www.abcam.cn/ps/products/54/ab54583/Images/ab54583-67510-anti-protein-casp-antibody-2a10-western-blot.jpg)
Western blot - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/ml + Tagged recombinant Protein CASP protein
Predicted band size: 77.5 kDaThis antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Expected MW 38 kDa.
This image was generated using the ascites version of the product.
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![Sandwich ELISA - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)](https://www.abcam.cn/ps/products/54/ab54583/Images/ab54583-212605-anti-protein-casp-antibody-2a10-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583)Detection limit of ab54583 is approximately 0.03 ng/ml as a capture antibody.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (24)
ab54583 被引用在 24 文献中.
- Kettunen P et al. SARS-CoV-2 Infection of Human Neurons Is TMPRSS2 Independent, Requires Endosomal Cell Entry, and Can Be Blocked by Inhibitors of Host Phosphoinositol-5 Kinase. J Virol 97:e0014423 (2023). PubMed: 37039676
- Fame RM et al. Age-appropriate potassium clearance from perinatal cerebrospinal fluid depends on choroid plexus NKCC1. Fluids Barriers CNS 20:45 (2023). PubMed: 37328833
- Papes F et al. Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nat Commun 13:2387 (2022). PubMed: 35501322
- Schilders KAA et al. Identification of SOX2 Interacting Proteins in the Developing Mouse Lung With Potential Implications for Congenital Diaphragmatic Hernia. Front Pediatr 10:881287 (2022). PubMed: 35615634
- Rosebrock D et al. Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells. Nat Cell Biol 24:981-995 (2022). PubMed: 35697781
