Anti-Protein C抗体(ab131251)
Key features and details
- Rabbit polyclonal to Protein C
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Protein C抗体
参阅全部 Protein C 一抗 -
描述
兔多克隆抗体to蛋白C -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to a sequence at the C terminal of Human Protein C.
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阳性对照
- Jurkat, CEM, SMMC and Hela cell lysates Flow Cyt (Intra): A549 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131251于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use 1-3µg for 106 cells.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 52 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
Use 1-3µg for 106 cells. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 52 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845). -
组织特异性
Plasma; synthesized in the liver. -
疾病相关
Thrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻译后修饰
The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. -
细胞定位
Secreted. Golgi apparatus. Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 5624 Human
- Omim: 612283 Human
- SwissProt: P04070 Human
- Unigene: 224698 Human
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别名
- Activation peptide antibody
- Anticoagulant protein C antibody
- APC antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody (ab131251)
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Overlay histogram showing A549 cells stained with ab131251 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with ab131251 1μg/1x106 cells for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG 5-10 μg/1x106 cells was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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All lanes : Anti-Protein C antibody (ab131251) at 0.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : CEM cell lysate
Lane 3 : SMMC cell lysate
Lane 4 : Hela cell lysate
Predicted band size: 52 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab131251 尚未被引用在任何文献中。