重组Anti-Proteasome subunit alpha type 6抗体[S01-4I2] (ab317398)
![Western blot - Anti-Proteasome subunit alpha type 6 Antibody [S01-4I2] (ab317398)](https://www.abcam.cn/ps/products/317/ab317398/Images/ab317398-536531-anti-proteasome-subunit-alpha-type-6-antibody-s01-4i2-western-blot-k562-c6-3t3-hela-human-rat-mouse.jpg "Western blot - Anti-Proteasome subunit alpha type 6 Antibody [S01-4I2] (ab317398)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [S01-4I2] to Proteasome subunit alpha type 6
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-Proteasome subunit alpha type 6抗体[S01-4I2]
参阅全部 Proteasome subunit alpha type 6 一抗 -
描述
兔单克隆抗体[S01-4I2] to Proteasome subunit alpha type 6 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Proteasome subunit alpha type 6.
Database link: P60900 -
阳性对照
- WB: K562, C6, NIH/3T3 and HeLa cell lysate. ICC/IF: HeLa cells.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
S01-4I2 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317398于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
|
|
| ICC/IF |
1/50.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 27 kDa. |
|
ICC/IF
1/50. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
序列相似性
Belongs to the peptidase T1A family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 5687 Human
- Entrez Gene: 26443 Mouse
- Entrez Gene: 29673 Rat
- Omim: 602855 Human
- SwissProt: P60900 Human
- SwissProt: Q9QUM9 Mouse
- SwissProt: P60901 Rat
- Unigene: 446260 Human
see all -
别名
- 27 kDa prosomal protein antibody
- IOTA antibody
- Macropain iota chain antibody
see all
图片
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Western blot - Anti-Proteasome subunit alpha type 6 Antibody [S01-4I2] (ab317398)All lanes : Anti-Proteasome subunit alpha type 6 Antibody [S01-4I2] (ab317398) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 27 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit alpha type 6 Antibody [S01-4I2] (ab317398)](https://www.abcam.cn/ps/products/317/ab317398/Images/ab317398-536530-anti-proteasome-subunit-alpha-type-6-antibody-s01-4i2-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit alpha type 6 Antibody [S01-4I2] (ab317398)Immunocytochemistry of Proteasome subunit alpha type 6 (green) in HeLa using ab317398 at dilution 1/20, and DAPI (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317398 尚未被引用在任何文献中。