AB109377

重组Anti-Proteasome subunit alpha type 6抗体[EPR5835]

Name: Proteasome subunit alpha type 6抗体[EPR5835] (ab109377)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109377
Price: 2327 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Proteasome subunit alpha type 6 antibody [EPR5835]

RabMAb
Recombinant
了解详情

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal Proteasome subunit alpha type 6 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

查看别名

PROS27, PSMA6, Proteasome subunit alpha type-6, 27 kDa prosomal protein, Macropain iota chain, Multicatalytic endopeptidase complex iota chain, Proteasome iota chain, Proteasome subunit alpha-1, PROS-27, p27K, alpha-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (AB109377)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (AB109377)

ab109377 at a 1/250 dilution staining Proteasome 20S alpha 6 in paraffin embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (AB109377)

All lanes:

Western blot - Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (ab109377) at 1/50000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Proteasome subunit alpha type 6 antibody [EPR5835] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5835

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome subunit alpha type 6 also known as PSMA6 is a component of the 20S core particle of the proteasome complex. This protein has a molecular mass of approximately 28.4 kDa. PSMA6 occurs in the cytoplasm and nucleus of eukaryotic cells. The proteasome functions as a protease that degrades unneeded or damaged proteins by proteolysis a chemical reaction that breaks peptide bonds. PSMA6 plays a role in the assembly and structural integrity of the proteasome.

Biological function summary

The proteasome is essential for the ubiquitin-proteasome pathway which regulates protein turnover by degrading ubiquitinated proteins. As part of the 20S core complex PSMA6 contributes to unfolding and translocating substrates into proteolytic sites. This activity helps control various cellular processes such as cell cycle progression and transcription. Abnormalities in this protein can affect normal cellular functions and regulate apoptosis and DNA repair.

Pathways

Proteasome subunit alpha type 6 is important in the Wnt signaling pathway and the NF-kB signaling pathways. In the Wnt pathway the proteasome controls the degradation of β-catenin an important effector in this signaling cascade. In the NF-kB pathway it regulates the turnover of IκB proteins repressors of NF-kB transcription factors. Together these pathways play roles in cellular proliferation differentiation and inflammation.

Abnormalities in PSMA6 have been associated with disorders such as cancer and neurodegenerative diseases. In cancer dysregulation of proteasome activity can lead to uncontrolled cell growth and resistance to apoptosis. Correlations exist between proteasome function and proteins like p53 a tumor suppressor protein. In neurodegenerative diseases like Alzheimer's proteasome impairment affects protein aggregation and degradation of misfolded proteins. Understanding PSMA6's role contributes to therapeutic developments in these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information PSMA6

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Gene 769:145228 PubMed33096182

2020

The potential role of PSMA6 in modulating fat deposition in pigs by promoting preadipocyte proliferation and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Qiangqiang Chen,Liaoyi Xu,Jianfeng Cai,Jinzhi Zhang

Electrophoresis 40:1731-1739 PubMed31021001

2019

Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly-horseradish peroxidase (PolyHRP).

Applications

Unspecified application

Species

Unspecified reactive species

Manish Mishra,Shuchita Tiwari,Anita Gunaseelan,Dongyang Li,Bruce D Hammock,Aldrin V Gomes

Analytical biochemistry 575:44-53 PubMed30914243

2019

Ponceau S waste: Ponceau S staining for total protein normalization.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Sander,Samantha Wallace,Rachel Plouse,Shuchita Tiwari,Aldrin V Gomes

Oncotarget 8:2233-2248 PubMed27903980

2016

Identification of biomarker microRNAs for predicting the response of colorectal cancer to neoadjuvant chemoradiotherapy based on microRNA regulatory network.

Applications

Species

Human

Yaqun Zhu,Qiliang Peng,Yuxin Lin,Li Zou,Peipei Shen,Feifei Chen,Ming Min,Li Shen,Jiajia Chen,Bairong Shen

International journal of cardiology 223:923-935 PubMed27589040

2016

Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts.

Applications

Species

Mouse

Rajeshwary Ghosh,Sumanta K Goswami,Luis Felipe B B Feitoza,Bruce Hammock,Aldrin V Gomes

PloS one 10:e0135392 PubMed26287535

2015

Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS).

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer E Gilda,Rajeshwary Ghosh,Jenice X Cheah,Toni M West,Sue C Bodine,Aldrin V Gomes

Investigative ophthalmology & visual science 55:4682-90 PubMed24994871

2014

Decreased proteasomal activity causes photoreceptor degeneration in mice.

Applications

Unspecified application

Species

Mouse

Ryo Ando,Kousuke Noda,Utano Tomaru,Mamoru Kamoshita,Yoko Ozawa,Shoji Notomi,Toshio Hisatomi,Mika Noda,Atsuhiro Kanda,Tatsuro Ishibashi,Masanori Kasahara,Susumu Ishida

View all publications

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重组Anti-Proteasome subunit alpha type 6抗体[EPR5835]