AB97563

Anti-Proteasome subunit alpha type 6抗体

Name: Proteasome subunit alpha type 6抗体 (ab97563)| Abcam中文官网
Brand: Abcam Limited
SKU: AB97563
Price: 4606 CNY
Availability: InStock

Anti-Proteasome subunit alpha type 6 antibody

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(1 Publication)

Rabbit Polyclonal Proteasome subunit alpha type 6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PSMA6 aa 50-250.

查看别名

PROS27, PSMA6, Proteasome subunit alpha type-6, 27 kDa prosomal protein, Macropain iota chain, Multicatalytic endopeptidase complex iota chain, Proteasome iota chain, Proteasome subunit alpha-1, PROS-27, p27K, alpha-1

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

ab97563, at a 1/200 dilution, staining Proteasome subunit alpha type 6 in paraformaldehyde-fixed A431 cells by Immunofluorescence analysis. Right image is merged with a DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

ab97563, at a 1/100 dilution, staining Proteasome subunit alpha type 6 in paraffin-embedded H1299 xenograft by Immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue staining Proteasome subunit alpha type 6 with ab97563 at 1/500 dilution.

Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue staining Proteasome subunit alpha type 6 with ab97563 at 1/500 dilution.

Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Immunohistochemical analysis of paraffin-embedded mouse urinary bladder tissue staining Proteasome subunit alpha type 6 with ab97563 at 1/500 dilution.

Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Unknown

Western blot - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

12% SDS PAGE

All lanes:

Western blot - Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Lane 4:

HeLa whole cell lysate at 30 µg

Predicted band size: 27 kDa

false

Supplier Data

Western blot - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

All lanes:

Western blot - Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution

All lanes:

rat liver lysate at 50 µg

Predicted band size: 27 kDa

false

Supplier Data

Western blot - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

All lanes:

Western blot - Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution

All lanes:

mouse liver lysate at 50 µg

Predicted band size: 27 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human PSMA6 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

P60900

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Proteasome subunit alpha type 6 also known as PSMA6 is a component of the 20S core particle of the proteasome complex. This protein has a molecular mass of approximately 28.4 kDa. PSMA6 occurs in the cytoplasm and nucleus of eukaryotic cells. The proteasome functions as a protease that degrades unneeded or damaged proteins by proteolysis a chemical reaction that breaks peptide bonds. PSMA6 plays a role in the assembly and structural integrity of the proteasome.

Biological function summary

The proteasome is essential for the ubiquitin-proteasome pathway which regulates protein turnover by degrading ubiquitinated proteins. As part of the 20S core complex PSMA6 contributes to unfolding and translocating substrates into proteolytic sites. This activity helps control various cellular processes such as cell cycle progression and transcription. Abnormalities in this protein can affect normal cellular functions and regulate apoptosis and DNA repair.

Pathways

Proteasome subunit alpha type 6 is important in the Wnt signaling pathway and the NF-kB signaling pathways. In the Wnt pathway the proteasome controls the degradation of β-catenin an important effector in this signaling cascade. In the NF-kB pathway it regulates the turnover of IκB proteins repressors of NF-kB transcription factors. Together these pathways play roles in cellular proliferation differentiation and inflammation.

Abnormalities in PSMA6 have been associated with disorders such as cancer and neurodegenerative diseases. In cancer dysregulation of proteasome activity can lead to uncontrolled cell growth and resistance to apoptosis. Correlations exist between proteasome function and proteins like p53 a tumor suppressor protein. In neurodegenerative diseases like Alzheimer's proteasome impairment affects protein aggregation and degradation of misfolded proteins. Understanding PSMA6's role contributes to therapeutic developments in these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

See full target information PSMA6

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Science signaling 9:ra86 PubMed27577261

2016

Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages.

Applications

Species

Unspecified reactive species

Anna M Piccinini,Lorena Zuliani-Alvarez,Jenny M P Lim,Kim S Midwood

View all publications

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Anti-Proteasome subunit alpha type 6抗体

Anti-Proteasome subunit alpha type 6 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Western blot - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Western blot - Anti-Proteasome subunit alpha type 6 antibody (AB97563)

Western blot - Anti-Proteasome subunit alpha type 6 antibody (AB97563)