Anti-Proteasome Activator Subunit 4/PSME4 抗体 [EPR13577(B)] - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (AB181203)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling unpurified Proteasome Activator Subunit 4/PSME4 with ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (AB181203)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Proteasome Activator Subunit 4/PSME4 with unpurified ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (AB181203)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Proteasome Activator Subunit 4/PSME4 with purified ab181203 at 1 : 1000 dilution (0.59 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Supplier Data

Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (AB181203)

All lanes:

Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 1/1000 dilution

Lane 1:

K562 cell line lysate at 20 µg

Lane 2:

HeLa cell line lysate at 20 µg

Lane 3:

HepG2 cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 211 kDa

false

• WB

Unknown

Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (AB181203)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 0.6 µg/mL

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Lane 2:

RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 211 kDa

false