Anti-Proteasome 26S S3/PSMD3抗体(ab3316)
Key features and details
- Rabbit polyclonal to Proteasome 26S S3/PSMD3
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-Proteasome 26S S3/PSMD3抗体
参阅全部 Proteasome 26S S3/PSMD3 一抗 -
描述
兔多克隆抗体to Proteasome 26S S3/PSMD3 -
宿主
Rabbit -
特异性
Detects proteasome 26S subunit S3. -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Drosophila melanogaster, Non human primates
-
免疫原
Synthetic peptide corresponding to Human Proteasome 26S S3/PSMD3 aa 513-534.
Sequence:EREQQDLEFAKEMAEDDDDSFP
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 20 µg/ml.
|
|
| ICC/IF |
1/50 - 1/500.
|
| 说明 |
|---|
|
Flow Cyt
Use a concentration of 1 - 20 µg/ml. |
|
ICC/IF
1/50 - 1/500. |
靶标
-
功能
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
序列相似性
Belongs to the proteasome subunit S3 family.
Contains 1 PCI domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 504937 Cow
- Entrez Gene: 5709 Human
- Entrez Gene: 22123 Mouse
- SwissProt: Q2KJ46 Cow
- SwissProt: O43242 Human
- SwissProt: P14685 Mouse
- Unigene: 12970 Human
- Unigene: 12194 Mouse
-
别名
- 26S proteasome non ATPase regulatory subunit 3 antibody
- 26S proteasome non-ATPase regulatory subunit 3 antibody
- 26S proteasome regulatory subunit RPN3 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
-
Flow Cytometry - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)Flow Cytometry analysis of HeLa cells labelng Proteasome 26S S3/PSMD3 with ab3316 (Pink) or a rabbit IgG isotype control (Black) 10 µg/mL. Goat anti-Rabbit IgG (H+L) Superclonal™ Alexa Fluor® 647 conjugate at a dilution of 1/50 was used as the Secondary Antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
-
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
数据表及文件
-
Datasheet download
文献 (1)
ab3316 被引用在 1 文献中.
- Li S et al. Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability. Nat Mater 16:953-961 (2017). PubMed: 28783156
