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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-Proteasome 26S S3/PSMD3抗体(ab3316)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
  • Flow Cytometry - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)

Key features and details

  • Rabbit polyclonal to Proteasome 26S S3/PSMD3
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Proteasome 26S S3/PSMD3抗体
    参阅全部 Proteasome 26S S3/PSMD3 一抗
  • 描述

    兔多克隆抗体to Proteasome 26S S3/PSMD3
  • 宿主

    Rabbit
  • 特异性

    Detects proteasome 26S subunit S3.
  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Cow, Drosophila melanogaster, Non human primates
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 26S S3/PSMD3 aa 513-534.
    Sequence:

    EREQQDLEFAKEMAEDDDDSFP

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3316于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use a concentration of 1 - 20 µg/ml.
ICC/IF
1/50 - 1/500.
说明
Flow Cyt
Use a concentration of 1 - 20 µg/ml.
ICC/IF
1/50 - 1/500.

靶标

  • 功能

    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • 序列相似性

    Belongs to the proteasome subunit S3 family.
    Contains 1 PCI domain.
  • Target information above from: UniProt accession O43242 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 504937 Cow
    • Entrez Gene: 5709 Human
    • Entrez Gene: 22123 Mouse
    • SwissProt: Q2KJ46 Cow
    • SwissProt: O43242 Human
    • SwissProt: P14685 Mouse
    • Unigene: 12970 Human
    • Unigene: 12194 Mouse
    • 别名

      • 26S proteasome non ATPase regulatory subunit 3 antibody
      • 26S proteasome non-ATPase regulatory subunit 3 antibody
      • 26S proteasome regulatory subunit RPN3 antibody
      • 26S proteasome regulatory subunit S3 antibody
      • OTTHUMP00000164347 antibody
      • P58 antibody
      • Proteasome (prosome, macropain) 26S subunit non ATPase 3 antibody
      • Proteasome subunit p58 antibody
      • PSMD3 antibody
      • PSMD3_HUMAN antibody
      • RPN3 antibody
      • S3 antibody
      • Tissue specific transplantation antigen 2 antibody
      • TSTA2 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
      Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)

      Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Flow Cytometry - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
      Flow Cytometry - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
      Flow Cytometry analysis of HeLa cells labelng Proteasome 26S S3/PSMD3 with ab3316 (Pink) or a rabbit IgG isotype control (Black) 10 µg/mL. Goat anti-Rabbit IgG (H+L) Superclonal™ Alexa Fluor® 647 conjugate at a dilution of 1/50 was used as the Secondary Antibody.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
      Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)

      Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)
      Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 26S S3/PSMD3 antibody (ab3316)

      Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3/PSMD3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab3316?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3316 被引用在 1 文献中.

    • Li S  et al. Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability. Nat Mater 16:953-961 (2017). PubMed: 28783156

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